Expression Of PTEN,P-Akt And P-ERK Protein In Gastric Carcinoma Tissues And Their Clinical Significance

Objective:To investigate the expression of PTEN,P-Akt and P-ERK protein in gastric carcinoma tissues and relationship between their expression and clinicalpathology parameters,and possible correlation,and to provide experimental clue for target treatment of gastric carcinoma simultaneously.Methods:Immunohistochemical assay was used to detect expressions of PTEN,P-Akt and P-ERK in 65 gastric cancer and adjacent tissues.Results:(1)The positive rate of PTEN was significantly lower in gastric cancer 58.46%(38/65) than that in normal gastric tissues100%(65/65),P=0.000;its expression positive rate was 77.78%(21/27)in well or moderately differentiated and 44.74%(17/38)in poorly-differentiated gastric carcinoma tissue,and the difference between them was distinct ,P =0.006. its expression positive rate was 77.27%(17/22)in gastric carcinoma tissue that the invasive deepth was not beyond placenta percreta and 48.84%(21/43)that beyond placenta percreta,the difference between them was distinct,P =0.019. its expression positive rate was 48.78%(20/41)in gastric carcinoma tissue following lymphatic metastasis and 75.00%(18/24)without lymphatic metastasis,the difference between them was distinct,P =0.025. its expression positive rate was 80.00%(16/20)in gastric carcinoma tissue in TNM(â… +â…¡)period and 48.89%(22/45)in TNM(â…¢+â…£)period,the difference between them was distinct,P =0.014. (2)The positive rate of P-Akt was significantly higher in gastric cancer67.69%(44/65)than that in normal gastric tissues26.15%(17/65),P=0.000;its expression positive rate was 80.49%(33/41)in gastric carcinoma tissue following lymphatic metastasis and 45.83%(11/24)without lymphatic metastasis,the difference between them was distinct,P =0.004.its expression positive rate was 70.37%(19/27)in well or moderately differentiated and 65.79% ( 25/38 ) in poorly-differentiated gastric carcinoma tissue,and the difference between them was not distinct,P =0.198.its expression positive rate was 63.64%(14/22)in gastric carcinoma tissue that the invasive deepth was not beyond placenta percreta and 69.77%(30/43)that beyond placenta percreta,the difference between them was not distinct,P =0.193. its expression positive rate was 60.00%(12/20)in gastric carcinoma tissue in TNM(â… +â…¡)period and 71.11%(32/45)in TNM(â…¢+â…£)period,the difference between them was not distinct,P =0.151. (3) The positive rate of P-ERK was significantly higher in gastric cancer 86.15%(56/65)than that in normal tissues16.92%(11/65),P=0.000;its expression positive rate was 68.18%(15/22)in gastric carcinoma tissue that the invasive deepth was not beyond placenta percreta and 95.35%(41/43)that beyond placenta percreta,the difference between them was distinct,P =0.005. its expression positive rate was 95.12%(39/41)in gastric carcinoma tissue following lymphatic metastasis and 70.83%(17/24)without lymphatic metastasis,the difference between them was distinct,P =0.009. its expression positive rate was 65.00%(13/20)in gastric carcinoma tissue in TNM(â… +â…¡)period and 95.56%(43/45)in TNM(â…¢+â…£)period, the difference between them was distinct,P =0.002. its expression positive rate was 77.78%(21/27)in well or moderately differentiated and 92.11%(35/38)in poorly-differentiated gastric carcinoma tissue,and the difference between them was not distinct , P=0.078. (4)PTEN was shown to correlate negatively with P-AKT(P=0.000) and P-ERK(P=0.005) expression.Conclusion:All of PTEN,P-Akt and P-ERK proteins expressions were related with the development,progress,invasiveness and metastasis of gastric carcinoma; The decrease or deletion of PTEN expression may be related to the abnormal activation of P-Akt and P-ERK protein,so it may not be able to effectively inhibit the Akt and the ERK signaling pathway,which play an important role in the initiation and development of human gastric cancer;The excessive expression of protein P-Akt and P-ERK in carcinoma tissue may be as the target point for molecular treatment of gastric carcinoma.