The Study On Relationship Between VEGF,PIGF And Angiogenesis In Retinoblastoma

Background and objective: Retinoblastoma is the most common intraocular neoplasm in baby and child,the morbidity rate is about 1?15000~1?28000,it has a high death rate about 5% [1].The invasiveness proliferation and the metastasis of the tumor both rely on the angiogenesis of tumor, Angiogenesis is a hot spot of the cancer research because of the significant effect on invasiveness proliferation and metastasis of tumor.The previous study showed that VEGF(Vascular endothelial growth factor) plays a key role during the growth of the tumor.While the PIGF(placenta growth factor),as a new member of the VEGFs,was expressed in many tumors. Few researches on PIGF in retinoblastoma were reported although the functions of the VEGF have been studied many years.In this paper,the study focuses on the expression of the VEGF and PIGF in tissues of retinoblastoma.The results show that PIGE has an important significance in directing the anti-angiogenesis therapy and raising the survival rates.Materials and methods:1.Materials:30 samples of the retinoblastoma adenocarcinoma come from the General Hospital of Shenyang Military Command and the First Hospita of China Medical University during 1965 to 2005 with the age from 0.5 to 6,are 18 male and 12 female.The section are recut for 4μm section and stained with routine HE.2.Methods:Immunohistochemistry : polyclonal anti PIGF was purchased fromSantaCruz,monoclonal antibody of CD34,VEGF were purchased from Shanghai changdao Company,Streptavidin / Peroxidase(SP)perform according to standard protocol except that the primary antibody were CD34,VEGF,P1GF.Immunohistochemistry was performed according to protocol of ABC Kit.3.Evolution3.1 Evolution of the MVD:The microvessel was labeled by CD34.The stained capillary vessel and microvessel were accounted according to the standard of the Weidner[1] .Any individual brown endothelial cell and cell cluster were account as one while the area of the hemocoele higher than 8 times of diameter of the red cells should be ignored.The methods of count: Count the CD34 positive microvessels under 200×.Four different views were selected random for counting the microvessels.The mean value is the MVD(number/x200)3.2 Evaluation of VEGF and PIGF:Positive VEGF expression defined as brown particles existed in cytoplasm and membrane.3.3 Statistical analysis:All statistical analyses were performed using the Statistical Package for SPSS ll.5.The association of variables was evaluated using theχ~2 test,t-test and Spearman analysis.Results:VEGF was expressed in 66.67%(20/30) of the retinoblastoma. The expression of VEGF in retinoblastoma which has invaded the optic nerve or not was significant difference(P < 0.05). The MVD of VEGF positive retinoblastoma was 36.2±2.4/oil lens,and 21.1±3.2/oil lens in VEGF negative tissues.It has significant difference (P<0.01).The MVD of PIGF positive intestines carcinoma was 33.1±2.4/oil lens and 30.8±2.6/oil lens in PIGF negative tissues.It has significant of difference(P<0.01).The MVD PIGF,VEGF is significant association with retinoblastoma Duke's stages and the translocation of lymph. The immunohistochemistry results showed that the MVD in non-differentiated neuroblastoma was significant higher than differentiated neuroblastoma , it has significant difference (P <0.01).The association of variables analysis showed that VEGF expression and the MVD value was significantly positive correlation(r=0.541,P<0.01),while PIGF expression and MVD value was also significantly positive correlation(r=0.392,P<0.01).Conclusion: 1.The expression of VEGF in two type retinoblastoma was significant difference.2.The expression of PIGF in two type retinoblastoma was significant difference.3.The expression of VEGF in retinoblastoma which has invaded the optic nerve or not was significant difference.4.VEGF and PIGF are significantly positive coordinated to angiogenesis in retinoblastoma,especially in non-differentiated neuroblastoma.This provides a new methods using vascular endothelial growth inhibitor to theat retinoblastoma and raise the survival rate.