The Research On Intervention And Mechanism Of Inflammatory Joint Injury Rats

Objective:Observing the effects of huwentoxin -I(HWTX-I) on activity of SOD,GSH-Px,CAT and the content change of MDA in serum,and on mRNA expression of inflammatory factor of IFN-γand PDGF,and apoptosis associated factor of bax and bcl-2 in ankle of adjuvant arthritis rats,compared with Ibuprofen,we evaluate the role of Huwentoxin-I(HWTX-I),a new type antiinflammatory medication,and the mechanisms of action on AA rat paw.Methods:88 grown-up male SD rats,which were randomly divided into 6 groups:the control group(n=8),the nonadministration group(n=16),the HWTX-I 15μg/kg group(n=16) the HWTX-I 30μg/kg group(n=16),the HWTX-I 60μg/ kg group(n=16),the Ibuprofen group(n=16).Rats serum of each group were detected activity of SOD,GSH-Px,CAT and the content change of MDA,eight rats of the model group,the Ibuprofen group,the three HWTX-I group were used to observe the expression of inflammatory factor of IFN-γand PDGF and apoptosis correlation factor Bax,bcl-2 protein,and another eight were used to observe morphological change.All rats were used to set up the adjuvant arthritis model combined with the method of inserting anaesthesic tube into Spinal epidural space on the study.Results:1.Compared with the non- administration group,the SOD activity of serum of HWTX-I three group raised 17.9%,24.3%,29.5%,and that of Ibuprofen group raised 13.4%,difference is conspicuously(p<0.05);Compared with Ibuprofen group,the SOD activity of serum of 30,60μg/kg HWTX-I each raised 9.7%(p<0.05) and 14.2%(p<0.01)2.Compared with the non- administration group,the CAT activity of serum of 60μg/kg HWTX-I group raised obviously (p<0.05),the increase of that of Ibuprofen group had no statistics sense;As CAT activity of serum,only 60μg/kg HWTX-I group of three groups is higher than Ibuprofen group (p<0.05).3.Compared with the non-administration group,the OSH-Px activity of serum of 30μg/kg HWTX-I raised 24.4%(p<0.05),and that of 60μg/kg HWTX-I group raised 56.8%(p<0.01),and that of Ibuprofen group raised 25.0%(p<0.05);Compared with Ibuprofen group,the GSH-Px activity of serum of only 60μg/ kg HWTX-I group of three groups increased conspicuously (p<0.05). 4.Compared with the non-administration group,the content of MDA of serum of 30μg/kg HWTX-I decreased 8.9%(p<0.05), and that of 60μg/kg HWTX-I decreased 23.6%(p<0.01),and that of Ibuprofen group reduced 10.0%(p<0.05);Compared with Ibuprofen group,the content of MDA of serum of 60μg/kg HWTX-I reduced 15.1%(p<0.05).5.The expression of Bax mRNA in the rats' ankle of HWTX-I 60μg/kg group is obviously less than the model group and Ibuprofen group by RT-PCR(p<0.05),nevertheless the expression of bci-2 mRNA in the rats' ankle of HWTX-I 60μg/kg group is obviously more than the model group and Ibuprofen group by RT-PCR(p<0.05).6.The expression IFN-γof mRNA in the rats' ankle of HWTX-I 60μg/kg group is obviously less than the model group and Ibuprofen group by RT-PCR(p<0.05),nevertheless the expression of PDGF mRNA in the rats' ankle of HWTX-I 60μg/kg group is obviously more than the model group and Ibuprofen group by RT-PCR(p<0.05).Conclusion:1.The administration of HWTX-I in Spinal epidural space can obviously relieve the damage of free radical as arthritis occurring. 2.The administration of HWTX-I in Spinal epidural space can decreased the expression of apoptosis factor-Bax mRNA, increase the expression of anti-apoptosis factor-bcl-2 mRNA and decreased the expression of inflammatory factor-IFN-γmRNA,increase the expression of anti- inflammatory factor-PDGF mRNA in the ankle of adjuvant arthritis rats,so that It suggest that the HWTX-I can heal arthritis through acting on JAK/STAT pathway and mitochondria pathway.3.the effect of HWTX-I on arthritis is prior to Ibuprofen.