The Study Of Daihuang Zhechong On Proliferation Of Hysteromyoma Cells And Stimulating Factor Which Can Improve Proliferation Of Hysteromyoma Cells

Objective:Set up experimental model of human uterine leiomyoma cells/ smooth muscle cells in vitro to research the effect of growth factor(IGF-I,EGF)on proliferation of hysteromyoma cells/ smooth muscle cells. Observe the action of serum containing Daihuang Zhechong on proliferation of hysteromyoma cells and stimulating factor which can improve proliferation of hysteromyoma cells based on serμm pharmacological methods to probe into the mechanism of action of Daihuang Zhechong on hysteromyoma.Methods:Culture human isolated uterine leiomyoma cells/ smooth muscle cells and detect the optical density of uterine leiomyoma cells/ smooth muscle cells which are stimulated with subjects on 12h,24h,48h,72h with the method of MTT.Results:1. IGF-I,EGF had an action of promoting proliferation of hysteromyoma cells.2. Serum containing Daihuang Zhechong showed an action of suppression on proliferation of uterine leiomyoma cells.3. Serum containing Daihuang Zhechong could antagonize the effect of IGF-I,EGF on proliferation of uterine leiomyoma cells.Conclusion:1.Serum containing Daihuang Zhechong showed a direct action of suppression on proliferation of hysteromyoma cells,which indicated hysteromyoma cells were target cells.2.Serum containing Daihuang Zhechong could antagonize the effect of IGF-I,EGF on proliferation of uterine leiomyoma cells.3.Suppressing proliferation of uterine leiomyoma cells and antagonizing the effect of IGF-I,EGF was the mechanism of action of Daihuang Zhechong and indicated the function of improving blood circulation and removing blood stasis and resolving nodes and masses of that.