Histology Research Of Vip Anti-rat Liver Fibrosis And The Effects On The Expressions Of α-SMA,TGF-β1,TNF-α

OBJECTIVE:Adopt Chemical hepatic fibrosis induced by CCL4 in rats To investigate the anti-fibrotic and hepatocyte-protective effects of VIP and to elucidate its possible mechanism.MOTHODS:Fivety male SD rats were randomized into two study groups :nomal contrast group ,model group, model group received intraperitoneal injection of 40% CCl4 Peanut oil 0.3ml/100g once every four days for 8weeks.Then nomal contrast group was named A group .model contrast group were randomized into four study groups: B:model contrast group, C:nature restoration group ,D:VIP 1nmol-treated group(VIP3.3ug/rat),E:VIP5nmol- Treated group(VIP16.5ug/rat),B,D,E group received Continues intra- peritoneal injection of 30% CCl4 Peanut oil 0.2ml/100g one time every four days for 20 days. Simultaneously Rats of VIP 1nmol-treated groups and VIP 5nmol-treated groups received intraperitoneal injection of VIP 3.3ug/rat and VIP16.5ug/rat one time every two days for 20 days.all rats were killed after 20 days, The levels of hydroxyproline in hepatic tissue were determinated by ultraviolet spectrophotometry.Collagen area percentage were detected by histopathology under optical microscopy and image analysis software. The expressions ofα-SMA,TGF-β1,TNF-αwere measured by immunochemical techniques and image analysis software.RESTULTS: Compared with model contrast group and nature restoration group, the levels of liver hydroxyproline and Collagen area percentage in VIP treated group had markedly decreased, VIP could reduced the collagen area and the expressions ofα-smooth muscle actin,Transforming growth factor-β1,and Tumor necrosis factor-α.CONCLUSION:VIP could slow down the progression of hepatic fibrosis.The mechanism of such effects may due to:(1) down-regulation the expressions ofα-smooth muscle actin,Transforming growth factor-β1, and Tumor necrosis factor-α.(2)the reduction injury of hepatocyte by adjust immune function of organism.(3)Suppress the activation of macrophage and Inflammation factor to hepatic stellate cell, Causes the activated hepatic stellate cell collagen secretion to reduce.