Effects Of Calming The Liver And Suppressing The Hyperactive Yang Therapy On Proteome In Migraine Rat Model With Hyperactivity Of Liver-Yang

Objective: To explore the molecule mechanisms of calming the liver and suppressing the hyperactive yang on migraine rat model with the hyperactivity of the liver-yang.Methods: Rat animal model of migraine with hyperactivity of liver-yang was established through electrical trigeminal ganglion stimulation, and by administering orally with aconite decoction. The total proteins of adrenal gland and splenic lymphocytes in the rats were separated by immobilized pH gradient-based two-dimensional gel electrophoresis (2-DE), and the 2-DE images were analyzed by PDQuest 7.0 software. Searching in the database was used to identify the differential proteins.Result: 1. There were 9 protein spots of adrenal glands expressing down-regulately and 5 up-regulately in the model group when compared to normal group. And they went back to normal lever in the therapy group. 8 differentially expressed proteins were identified.2. There were 16 protein spots of splenic lymphocytes expressing down-regulately and 4 up-regulately in the model group when compared to normal group. And they went back to normal lever in the therapy group. 10 differentially expressed proteins were identified.Conclusion: 1. Calming the liver and suppressing the hyperactive yang therapy can improve the headache of animals. 2. The differential proteins of adrenal gland and splenic lymphocytes in migraine rat model with the hyperactivity of the liver-yang and treated group had showed dynamic state.3. Annexin-A1,Peroxirdoxin-II,Heat shock protein-27 were supposed to correlated with hyperactivity of the liver-yang. Calming the liver and suppressing the hyperactive yang therapy may effect on migraine with the hyperactivity of the liver-yang by accommodating the express of multitude proteins.