Studies On The Antitumor Active Constituents From The Root Of Ardisia Crispa

Ardisia crispa,is one of the many Ardisia species used in folk medicine in the southeast of China.The roots of Ardisia crispa has been used as an anti-inflammatory/analgesic medication and to improve general blood circulation.Since 1970,there have been many records in the literature regarding the medicinal use or chemical constituents of many other species of Ardisia.However,Ardisia crispa. has hardly been phytochemically investigated till now.So in order to find bioactive constituents of the plant,systemically phytochemical research has been done.In our phytochemical research of the roots of Ardisia crispa., 13 compounds have been isolated and 11 of which have been identified by modem specrtroscopy.The compounds that have been identified are:Wogonin(1),Oroxylin A(2),Wogonoside(3),Baicalin(4), (+)Anwulignan(5),Meso-dihydroguaiaretic acid(6),4-Hydroxyvaleric acid(7),Bergenin(8),n-tetradecane(9),β-Sitosterol(10), Ardisiacrispin C(3β-O-{α-L-rhamnopyranosyl-(1→2)-β-D-glucopyrano -syl-(1→4)-[β-D-glucopyranosyl-]-α-L-arabinopyra-nosyl}-3β,16α,28-trihydroxy-olean-12en-30-oic acid 30-O-[3′α-D-glucopyranuronate-gly -cerol(1′→30)]ester)(11).Among these compounds,compound 11 are new,compound 2-7 and 9 were obtained from this genus for the first time,compound 10 was got from this Ardisia crispa for the first time.In Vitro anti-tumour activity(MTT based)of four compounds were evaluated on Bel-7402 cancer cell.Wogonoside and ardisiacrispin C(compound 11)showed potent inhibitory activity,which was stronger than 5-Fu.