Comparison Of Oxygen Carrying And Delivering Capacity And Survival Ability Of Shed Blood Salvaged From Field And Stored Concentrated Erythrocyte

Objective: To investigate the effect of intraoperative salvage of autoblood on oxygen carrying capacity and survival ability of erythrocytes, compare oxygen carrying capacity and survival ability of erythrocytes and that of periph erythrocytes, evaluate the quality of erythrocytes of salvaged autoblood comprehensively, provide the testing proof for the application feasibility and safety of intraoperative salvage of autoblood, and enhance the generalization and application of salvaged autoblood. To investigate the difference of intraoperative salvage of autoblood on oxygen carrying capacity and survival ability of erythrocytes, compare oxygen carrying capacity and survival ability of erythrocyte and that of stored variant guidance the application of clinic blood transfusion.Methods: Thirty ASAâ…¡orâ…¢patients aged 30-64yr undergoing surgical intervention were enrolled in this study. ZTTT-3000 cell saver system was used for intraoperative blood salvage. Thirty samples of blood salvaged from field and stored variant RBCs were taken. The pH, PO2, SPO2, and Na+ and K+ extra-cellular concentration of RBCs were detected. The P50 were calculated according to formula. Euzymelinked immunosorbebt assay, chemical method, freezing thawing method and spectrophotometer method were used to detect the 2-3-DPG concentration of RBCs, the activity of pyruvate kinase, the intra-cellular pH of RBCs and the intra-cellular concentration of RBCs, respectively.Result :1 There was no significant difference in P50, 2-3-DPG concentration of RBCs and activity of pyruvate kinase between salvaged RBCs and periph erythrocytes. The intra-and-extra-cellular pH of RBCs was significantly higher than that of periph RBCs (P<0.05). The intra-and-extra-cellular Na+ concentration of RBCs was significantly higher than that of periph erythrocytes (P<0.05). The intra-and-extra-cellular K+ concentration of RBCs was sigenificantly lower in salvaged RBCs than in periph erythocytes (P<0.05).2 P50 was significantly lower, the 2-3-DPG concentration and activity of pyruvate kinase, the intra-and-extra-cellular pH of RBCs was significantly higher, the intra-and-extra-cellular Na+ concentration of salvaged RBCs was significantly higher, the intra-and-extra-cellular K+ concentration was significantly lower in salvaged RBCs than in stored variant RBCs (P<0.05).Conclusion: There was no significant difference in oxygen carrying capacity and mean cell age of RBCs between salvaged BCs and periph erythrocytes. The oxygen carrying capacity of salvaged RBCs is better than that of stored variant RBCs and the salvaged RBCs is younger than that of stored variant RBCs.