Kinetics Of Red Blood Cell Carrying Oxygen And Storage Effect On Erythrocyte's Oxygen-carrying Function

The oxygen transmission in body is a complex but orderly dynamic procedure, so it is necessary to research and analyze the specific kinetic process of red blood cell carrying oxygen. With the preservation of the different conditions and save time, red blood cells may undergo a series of structural and functional changes,which may have significant impacts on the quality of blood and results of clinical blood transfusion. The study of oxygen transmission differences between "fresh" blood and red blood cells stored and storage effect on erythrocyte's oxygen-carrying function is very important. This research analyzes the oxygen-carrying capacity changes of red blood cell in the preservation process from both thermodynamics and kinetics; investigate the effects of save time and pre-processing operation on erythrocyte's oxygen-carrying function and blood properties.Objective:To study the dynamic process of red blood cells carrying/releasing oxygen, and construct evaluation system for erythrocyte oxygen-carrying function. The effects of save time and pre-processing operation on erythrocyte's oxygen-carrying function and blood properties were analyzed, so as to provide a scientific basis and guidance for blood preservation and scientific blood transfusion. Methods:Observing the changes of oxygen saturation (Sat) and the partial oxygen pressure (P02) with time in the red blood cell oxygen dissociation process, and analyzing data by curve fitting; detecting the oxygen carrying function and physical/chemical parameters in different conservation phase of whole blood and suspension RBC.Results:The curve of oxygen saturation over time(Sat-t) has the typical"S"curve characteristic. Building a more effective oxygen-carrying evaluation system by leading in new oxygen dissociation kinetic parameter T*50 and effective oxygen-carrying volume Q. The effective oxygen-carrying volume decreased with the preservation time, after 1 week, a decrease of 27.5% and 19.2% respectively. At the end of preservation, the effective oxygen-carrying volume of whole blood was only 39 percent of "new blood" and suspended RBC only retained 51% effective oxygen-carrying volume also. P50 values decreased in the preservation period, at the beginning decline for the first three weeks, but a significant change in the last week. PH, Na+ concentration was declined, K+ concentration gradually increased; free Hb increased and reached the initial value of 5 ~ 10 times at the end of preservation. Glucose gradually reduced with the extension of storage time, after saving 35 days, the glucose was close to 0; lactate dehydrogenase increase rapidly over time , and near 3-fold at last.Conclusion : Oxygen-carrying function can be described and evaluated systematically by considering thermodynamic parameter P50, oxygen dissociation kinetic parameter T*50 and the effective oxygen-carrying volume Q. Blood storage time and pre-processing operations have an important impact on erythrocyte's oxygen-carrying capacity and blood physical and chemical properties; filtration, centrifugation and other operations will result in a one-time injury in red blood cells.