| Objective: Many kinds of etiological factors can induce myocardial fibrosis(such as inflammation,ischemia,hypoxia), excessive collagen fibers accumulate in the structure of the normal myocardial tissue, the concentration of collagen in the myocardial tissue significantly increased or the composition of collagen changed. This pathological change exists in many kinds cardiovascular diseases, and myocardial fibrosis is the crucial period from compensated stage to decompensated stage. The loss of equilibrium between the myocardial tissue and interstitial substance ,and the hardness of the heart can be changed throught the improvement of myocardial fibrosis, and it is helpful to the restore function of heart.The chemical name of Emodin is 1.3.8-tri-hydroxy-6 methyl anthraquinone, Molecular formula is C15H10O5, Molecular Weight is 270.23. The chemical constitution belongs to class of the hydroxyanthraquinone. The Emodin is the effective component of the traditional Chinese medicine Rhubarb, has the widespread pharmacologicalaction: antiinflammatory, bacteriostasis, immunological regulation, protect liver and kidney, anti-tumor etc. The study in recent years shows that the Emdion has the function of anti-organ fibrosis, the study mostly concentrates on the aspect of the liver, kidney and pancreas. The influence of the myocardial fibrosis have not been reported. Through this study, we can discuss the possible mechanism of how the Emdion can benefit on the rats injured by the Isoproterenol, and provide possible theory for preventing and curing myocardial fibrosis in the way of integrated traditional and western medicine.Methods: 63 male Sprague-Dawley rats were randomly divided into three groups, there were 21 rats in each group. A: the Emodin treatment group(5mg·kg-1·d-1);B: the Model group(Isoproterenol,ISO); C: the Normal group. Rats in A and B group injected Iso for 7days through subcutaneous injection. Rats in C group injected Isotonic Na chloride for 7days through subcutaneous injection. Randomly selected a mouse from each group, take the specimen of the heart, observe pathological changes of myocardial tissue through light microscope after hematoxylin and eosin stain, the group of A and B show apparent myocardial fibrosis. The rats of A group were drenched with emodin for eighth weeks, B and C group were drenched equal volume isotonic Na chloride for eight weeks. Half of rats were killed to take blood and cardiac muscle example from each group at the fourth week and the eighth week to test the contents of NO, SOD, MDA , PGI2 and TXA2 in blood, and observe the changes of the LVW and LVI. After hematoxylin and eosin stain, observe pathological changes of myocardial tissue through light microscope. Observe changes of collagen fiber Hyperplasia through MASSON staining.Result:1.the changes of LVI in each groupCompare with normal group in the fourth week, the LVI level of the rats injected with ISO increases obviously (P<0.01). Compare with the Emodin group in the fourth week, the LVI level of the rats injected with ISO decreases obviously(P<0.01). Compare with ISO group in the eighth week, the LVI of the rats in normal group decreases obviously(P<0.01).Compare with ISO group in the eighth week, the LVI of the rats in Emodin group decreases obviously(P<0.01). Compare with the emodin group in the fourth week, the LVI level of the emodin group in the eighth week decreases obviously(P<0.01).The result show that the emodin can reduce the LVI level of the rat injuried by ISO and make the myocardial fibrosis improved.2.the changes of the NO level in each groupCompare with normal group in the fourth week, the NO level of the rats injected with ISO decreases obviously(P<0.01). Compare with the Emodin group in the fourth week, the NO level of the rats injected with ISO decreases obviously (P<0.01). Compare with in normal group group in the eighth week, the NO of the ISO the rats decreases obviously (P<0.05).Compare with ISO group in the eighth week, the NO of the rats in Emodin group increases obviously (P<0.01). There is no different between the NO level of the Emodin group in fourth week and eighth week.The result show that the emodin can increase the NO level of the rat injuried by ISO.3.The changes of the SOD level in each groupCompare with normal group in the fourth week, the SOD level of the rats injected with ISO decreases obviously(P<0.05). Compare with the Emodin group in the fourth week, the SOD level of the rats injected with ISO decreases obviously (P<0.05). Compare with in normal group group in the eighth week, the SOD of the ISO the rats decreases obviously (P<0.05).Compare with ISO group in the eighth week, the SOD of the rats in Emodin group increases obviously(P<0.05). Compare with the emodin group in the fourth week, the SOD level of the emodin group in the eighth week increases obviously(P<0.05).The result show that the emodin can induce the SOD level of the rat injuried by ISO, and make the myocardial fibrosis improved.4.The changes of the MDA level in each groupCompare with normal group in the fourth week, the MDA level of the rats injected with ISO increases obviously(P<0.01). Compare with the Emodin group in the fourth week, the MDA level of the rats injected with ISO increases obviously(P<0.05). Compare with in normal group group in the eighth week, the MDA of the ISO the rats decreases obviously (P<0.05).Compare with ISO group in the eighth week, the MDA of the rats in Emodin group increases obviously (P<0.05). Compare with the emodin group in the fourth week, the MDA level of the emodin group in the eighth week decreases obviously(P<0.05).The result show that the emodin can induce the MDA level of the rat injuried by ISO, and make the myocardial fibrosis improved.5.the changes of the PGI2 level in each groupCompare with normal group in the fourth week, the PGI2 level of the rats injected with ISO decreases obviously(P<0.01). Compare with the Emodin group in the fourth week, the PGI2 level of the rats injected with ISO decreases obviously(P<0.01). Compare with in normal group group in the eighth week, the PGI2 of the ISO the rats decreases obviously (P<0.01) .Compare with ISO group in the eighth week, the PGI2 of the rats in Emodin group increases obviously(P<0.05). There is no different between the PGI2 level of the Emodin group in fourth week and eighth week.The result show that the emodin can induce the PGI2 level of the rat injuried by ISO, and make the myocardial fibrosis improved.6.the changes of the TXA2 level in each groupCompare with normal group in the fourth week, the TXA2 level of the rats injected with ISO increases obviously(P<0.05). Compare with the Emodin group in the fourth week, the TXA2 level of the rats injected with ISO increases obviously(P<0.01). Compare with in normal group group in the eighth week, the TXA2 of the ISO the rats increases obviously (P<0.05).Compare with ISO group in the eighth week, the TXA2 of the rats in Emodin group decreases obviously (P<0.01). There is no different between the TXA2 level of the Emodin group in fourth week and eighth week.The result show that the emodin can induce the TXA2 level of the rat injuried by ISO and make the myocardial fibrosis improved.Conclution: 1.Large dose Isoproterenol can induce myocardial fibrosis in rats.2.EM can reduce myocardial fibrosis in rats induced by ISO through increasing the NO level in the blood serum.3.EM can reduce myocardial fibrosis in rats induced by ISO through increasing the SOD level and decreaseing the MDA level in the blood serum.4. EM can reduce myocardial fibrosis in rats induced by ISO through increasing the PGI2 level and decreasing the TXA2 level in the blood . |
