Expression And Significance Of WT-1, P53, MMP-9 And TIMP-2 In Ovarian Papillary Serous Carcinomas

Objective:To investigate the expression and significance of WT-1, P53, matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-2 (TIMP-2) in ovarian papillary serous carcinomas, and approach the relationship between the expression of these immuno- marker in ovarian papillary serous carcinomas and the degree of its differentiation or the metastasis. The expression-difference of these immuno- markers and significance were analyzed in ovarian papillary serous carcinomas, primary peritoneal papillary serous carcinomas and uterine papillary serous carcinomas.Methods: The expression of WT-1, P53, MMP-9 and TIMP-2 in 52 cases of ovarian papillary serous carcinomas ( 18 cases of well differentiation,8 cases of moderate differentiation and 26 cases of poor differentiation), 15 cases of primary peritoneal papillary serous carcinomas and 11 cases of uterine papillary serous carcinomaswere were studied by immunohistochemical S-P stain method. The difference of expression WT-1, P53, MMP-9 and TIMP-2 in each group of tumor were analyzed throught countting positive rate and image analysis. Results:1. 50 of 52 cases of ovarian papillary serous carcinomas were positive for expression of WT-1, positive expression rate was 96.2%; among of them, 46 cases was strong expression. 14 of 15 cases of peritoneal papillary serous carcinomas revealed strong expression of WT-1, positive expression rate was 93.3%, with strong intensity in 11 cases. 4 of 11 cases of uterine papillary serous carcinomas showed weakly positive reactions for WT-1, the positive expression rates was 36.4%.There was a significant difference for the expression rates of WT-1 in ovarian papillary serous carcinomas, peritoneal papillary serous carcinomas and uterine papillary serous carcinomas (P<0.05).The result of image analysis showec that the Area integrated optical density (AIOD) of WT-1 in ovarian papillary serous carcinomas and primary peritoneal papillary serous carcinomas were high than that in uterine papillary serous carcinomas (P<0.01).2. P53 was expressed in 41 of 52 of cases of ovarian papillary serous carcinomas, the positive expression rates was 78.8%. In it, 25 cases were strong expression, 5 cases were moderate expression, 11 cases showed weakly positive. The 12 of 15 cases of peritoneal papillary serous carcinomas showed positive for P53, the positive expression rates was 80.0%. Among of them, 6 cases were strong expression, 6 cases were weakly positive. P53 was expressed in 8 of 11 cases of uterine papillary serous carcinomas, the positive expression rates was 72.7%. In them, 4 cases were strong expression, 1 case showed moderate positive, 3 cases showed weakly positive.There was not a significant difference for the expression rates of P53 in ovarian papillary serous carcinomas, peritoneal papillary serous carcinomas and uterine papillary serous carcinomas (P>0.05).3. 47 of 52 cases of ovarian papillary serous carcinomas were positive for expression of MMP-9, positive rate was 90.4 %,among of them,30 cases were strong expression.,6 cases were moderate expression. 11 cases showed weakly positive. The 14 of 15 cases of peritoneal papillary serous carcinomas showed positive for MMP-9.The positive expression rates was 93.3%. In them, 8 cases were strong expression ,3 cases were moderate expression , 3 cases were weakly positive. All cases of uterine papillary serous carcinomas showed positive for MMP-9. The positive expression rates was 100%, In them, 7 cases were strong expression.,4 cases showed weakly positive .There was not a significant difference for the expression rates of MMP-9 in ovarian papillary serous carcinomas, peritoneal papillary serous carcinomas and uterine papillary serous carcinomas(P>0.05).4. TIMP-2 is expressed in 50 of 52 of cases of ovarian papillary serous carcinomas.The positive expression rates was 96.2%, among of them,38 cases were strong expression. 4 cases were moderate expression and 8 cases showed weakly positive. The 14 of 15 cases of peritoneal papillary serous carcinomas showed positive for TIMP-2.The positive expression rates was 93.3%. 11 cases were strong expression, 1 case showed moderate positive, 2 cases showed weakly positive. All cases of uterine papillary serous carcinomas showed positive for TIMP-2. The positive expression rates was 100%. In them, 9 cases were strong expression , 2 cases were moderate positive. There was not a significant difference for the expression rates of TIMP-2 in ovarian papillary serous carcinomas, peritoneal papillary serous carcinomas and uterine papillary serous carcinomas (P<0.05)5.The area integrated optical density (AIOD)of WT-1,P53,MMP-9 and TIMP-2 analyzed by image analyzer in low differentiation group of ovarian serous carcinomas were high than that in high differentiation group (P<0.01)Conclusions:1. The AIOD of WT-1 expressed in ovarian papillary serous carcinomas and primary peritoneal papillary serous carcinomas were higher than that in uterine papillary serous carcinomas (P<0.01). It indicated that the detection of WT-1 may be an useful marker which can distinguish ovarian papillary serous carcinomas from uterine papillary serous carcinomas.2. The overexpression of P53, MMP-9 and TIMP-2 were all appeared in most of ovarian papillary serous carcinomas, primary peritoneal papillary serous carcinomas and uterine papillary serous carcinomas. The difference of the positive rates and AIOD value did not show statistical significance. It indicated that the overexpression of WT-1, P53, MMP-9 and TIMP-2 are related to carcinogenesis and development of the ovarian serous carcinomas.3.The AIOD of WT-1,P53,MMP-9 and TIMP-2 analyzed by image analyzer in low differentiation group of ovarian serous carcinomas were higher than that in high differentiation group (P<0.01). It indicates that the overexpression of WT-1, P53, MMP-9 and TIMP-2 are related to degree of differentiation of the ovarian papillary serous carcinomas.