The Effect On The Content Of 5-HT And 5-HIAA In The Insomnia Rats' Hypothalamus Caused By Sishencong Acupuncture

Objective: 1. To observe the influence of spontaneous activity direct sleep rate the sleeping time of rats eoursed by sodium pentobarbltal and sleep rate eoursed by sodium pentobarbltal at the critical dose , explicit the effection of insomnia by Sishencong acupuncture. 2. To observe the content of 5-HT and 5-HIAA in the insomnia rats' Hypothalamus caused by Sishencong acupuncture, thus to reveal the central mechanism of the treatment for insomnia by acupuncture.Methods: 1. Thirty male Wistar rats are randomly divided into three groups: blank control group (n=10); hand acupuncture group (n=10); electro-acupuncture group (n=10 ) .By using SD-5G physiological and pharmacolo-gical experimental equipment record the spontaneous activity of each group after acupuncture . 2. Thirty male Wistar rats are respectively and randomly divided into three groups : blank control group (n=10); hand acupuncture group (n=10); electro-acupuncture group (n=10) .Observe the influence of spontaneous activity direct sleep rate the sleeping time of rats eoursed by sodium pentobarbltal and sleep rate eoursed by sodium pentobarbltal at the critical dose respectively.3.Fifty male Wistar rats are divided into five groups at random: Blank control group (n=10); Model control group (n=10); Diazepam group (n=10) .Hand acupuncture group(n=10); Electro-acupun- cture group(n=10); Duplicate insomnia model of rats by means of PCPA abdominal injection. The animals in the two acupuncture groups will be treated with 7 days' acupuncture, the animals in Diazepam group will be treated with seven days' diazepam intragastric administration, then study the results of the treatments by observing the content of 5-HT, 5-HIAA in the insomnia rats' Hypothalamus.Results: 1. Both hand acupuncture group and electro-acupunc- ture group can ruduce the spontaneous activity with comparison to control group(P<0.01), electro-acupuncture group reduce the spontaneous activity more obviously compared with hand acupuncture group (P<0.01) .2.There is no direct sleep rate both in hand acupunc- ture group and electro-acupuncture group. Hand acupuncture group and electro-acupuncture group can prolong the sleeping time of rats eoursed by sodium pentobarbltal and increase sleep rate eoursed by sodium pentobarbltal at the critical dose, with significance difference comparison to control group ( P<0.01 ) .Compared with hand acupuncture group, electro-acupuncture group has significance difference (P<0.01) . 3.After PCPA abdominal injection the normal sleep of rats disappeared, at the same time, the content of 5-HT and 5-HIAA in the Hypothalamus of rats decreased with comparison to blank control group (P< 0.05) . Model group compared with the blank, the content of 5-HT and 5-HIAA in the Hypothalamus of rats decreased obviously, has significance difference ( P<0.01). There is no significant change between Diazepam group and Model control group ( P>0.05) . Both hand acupuncture group and electro-acupuncture group can increase the content of 5-HT, 5-HIAA in the insomnia rats' Hypothalamus, with significance difference comparison to model control group (P<0.05), and have significance difference comparison to Diazepam group (P<0.01) . Compared with hand acupuncture group, electro-acupuncture group has signifi-cance difference (P<0.05) .Conclusions : 1. Sishencong acupuncture can ruduce the spontaneous activity, prolong the sleeping time of rats eoursed by sodium pentobarbltal and increase sleep rate eoursed by sodium pentobarbltal at the critical dose. Sishencong acupuncture has sedative and hypnotic effects, espencialy electro-acupuncture.2.Sishencong acupuncture has no obviously direct sleep effects on rats.3.Acupuncture can increase the content of 5-HT, 5-HIAA in the insomnia rats' Hypothalamus. This may be one of the mechanisms that acupuncture can promote sleep and enhance the quality of it.