Research Of FLT3/ITD Mutation In Acute Myelocytic Leukemia

Objective:To detect the mutation of FLT3/ITD in acute myelocytic leukemia(AML),observe the distribution of FLT3/ITD mutation in different subsets of AML,and to explore the relationship between the FLT3/ITD mutation and clinical feature of AML,so that to provid theory evidence for clinic diagnosis,prognosis evaluation and targeted therapy.Methods:The FLT3/ITD mutation was detected by PCR in 72 cases of AML.Results:1.The FLT3 gene from 72 cases of AML was performed by PCR,and the amplified products were analyzed by agarose gel electrophoresis:FLT3/ITD were detected in 20 of 72(27.8%)AML cases,including 2/6(33.3%)of M₁,4/10 (40%)of M₂,6/18(33.3%)of M₃,2/12(16.7%)of M₄,5/14(35%)of M₅,1/5 (20%)of M₆.2.The FLT3/ITD mutation has heterogeneity.3.Compares with the FLT3/ITD negative patients,FLT3/ITD masculine patients have higher count of peripheral blood leucocyte and higher percentage of marrow blast cells(P＜0.05)Conclusion: 1.The positive rate of the FLT3/ITD mutation in AML is 27.8%in this research.2.The method of PCR to detected FLT3/ITD was very easy,fast and cheap,and it may be used as a routine molecule detection technique in clinic.3.The AML patients with FLT3/ITD may have distinct clinic features,such as bad prognosis and easy to recur,and so on.To detect FLT3/ITD mutation could provide theory evidence for the diagnosis,earlier period direct interference or target therapy and prognosis prediction with AML.