Expression And Clinical Significance Of HIF-1α Receptor In Human Hepatocellular Carcinoma

Objective: To investigate the expression of HIF-1αin human primary hepatocellular carcinoma(HCC) and its relationship with the tumor pathological characteristics.Methods: Fresh tumor and normal liver tissues were obtained from 22 hepatocellular carcinoma patients after hepatectomy Expression levels of HIF-1αmRNA were quantitatively analyzed by real-time PCR. The protein level were analyzed by immunohistochemistry .Results: HIF-1αwas expressed in most hepatocellular carcinoma tissues,including sixteen cases .And low expression in normal liver tissues only two cases。The relative of HIF-1αexpression level in hepatocellular carcinoma liver tissues was higher than that in nomal tissues(P<0.05.The patient who has complete tumor capsule,venous invision,low differentiation express more HIF-1αmRNA .The expression of HIF-1αmRNA has no relationship with gender,age,cirrhosis nodus,tumor size,tumor number,AFP,carcinoma embolus,HBsAg .(p>0.05)Conclusion: Both the normal liver tissue and HCC tissue express HIF-1α, and it is probably affected by litlle oxcygen .It has relatoionship with tumor capsule,venous invision,differentiation。The expression of HIF-1αmRNA has no relationship with gender,age,cirrhosis nodus,AFP,tumor size,tumor number,HBsAg .