Effects And Mechanism Of Jin Dan Shui Wan On Bone Density, Blood Lipid Of The Hyperlipidemia Induced Osteoporosis Rats

Objective: Through observation of animal experiments on Prey's Jin Dan Shui Wan hyperlipidemia induced osteoporosis rat bone tissue morphology and BMP-2, ER expression of the organization and molecular biology point of view, to investigate the treatment of Prey Hyperlipidemia induced osteoporosis mechanism.Methods: This study will be 27 adult SD rats were randomly divided into three groups, a control group: gavage saline morning at 5 ml / kg; 2. Model: high-fat diet am gavage 5 ml / kg, saline pm gavage 5ml/kg; 3. Experimental groups: high-fat diet am gavage 5 ml / kg, gavage of the afternoon's Dan Jin Shui Wan water extract of 5 ml / kg. Before the start of experiments and experimental testing at the end of the four blood lipids: TG (total triglyceride), TC (total cholesterol), HDL-C (high-density lipoprotein cholesterol), LDL-C (low-density lipoprotein cholesterol); experiment At the end of measuring bone density, by HE staining, Jin Dan Shui Wan's observation of its bone tissue in the osteoblasts and osteoclasts of the impact of using immunization techniques, their bone tissue in BMP-2 (BMP Protein-2), ER (estrogen receptor) in the level of detection, in situ hybridization of bone tissue in the ER mRNA level to detect, investigate solutions to the prey's Jin Dan Shui Wan osteoporosis bone tissue in rats In the BMP-2, ER expression.Results: 1. Experimental group compared with the model group TG decreased significantly, HDL-C increased significantly, LDL-C decreased significantly, statistical analysis of a significant difference (p <0.01).2. Experimental group compared with the model group significantly increased bone mineral density (p <0.01), bone mineral density in the experimental group and control group had no significant difference (P> 0.05). 3. Experimental group compared with the model group increase in the number of osteoblasts (p <0.01), reducing the number of osteoclasts (p <0.01). 4. Immunohistochemistry of indicators suggest that the BMP-2 are mainly distributed in the osteoblast cell cytoplasm, ER mainly in the nuclei in the experimental group, compared with the model group, the bone tissue in the BMP-2, ER expression significantly increased (p <0.01 ). The ER mRNA level increased.Conclusion : 1. Hyperlipidemia induced osteoporosis. 2. Dan Jin Shui Wan's solution will enable Prey hyperlipidemia induced osteoporosis rat osteoblasts BMP-2, ER increased the expression level of BMP-2, ER synthesis, secretion. 3. Jin Dan's solution has hypolipidemic Shuiwan to improve the dual role of osteoporosis.