| Osteoporosis is a common disease and frequently occurring disease in the elderly. The treatment is still mainly based on drugs, but most of them are expensive, toxic and side effects and the effect is not exact. In recent years, the low frequency electromagnetic field therapy has been paid more and more attention to its non invasion, non damage, no toxic side effects and convenient use and so on. However, due to the existence of "window effect" and the mechanism of the electromagnetic field, the optimal treatment parameters are difficult to determine, leading to the clinical effect of the treatment. The subject group in previous studies have shown that 50 Hz different intensity of the pulse wave, sine wave and triangular wave electromagnetic field(triangular electromagnetic fields, TEMFs) can promote the osteogenic differentiation and maturation of cultured fibroblasts in vitro, and first reported the sine wave magnetic field can improve the growth of rats during the peak bone density bone mineral density(BMD). To explore other types of electromagnetic field is an anti osteoporosis activity and screening the best treatment parameters, the comparative study of the effect of 0.1mt, 0.6m T, 1.2m T, 1.8m T and 2.4m T 50 Hz TEMFs of one month old SD rats BMD, in order from find a group based TEMFs ideal electric magnetic field treatment parameters.The 60 only 1 month old female SD rats were randomly divided into 6 groups, with 10 rats in each group, which per day for 5 groups with 50 Hz with different intensity of TEMFs 90 min, group 1 as control group, every place the device in the same time in the same magnetic field occur, but is not energized. The total BMD was measured once a month. After 3 months, all the animals were killed and the important organs were taken for safety evaluation. The blood and bone tissues were taken for analysis. The results were as follows:1. The total body BMD and the BMD of the in the body of the 0.1m T group were significantly higher than that in the control group, while the vertebral body BMD was higher than the control group, but there was no statistical significance. Compared with the control group, there was no significant difference between the other groups.Results of three point bending test showed that the elastic modulus, maximum load and yield strength of the 0.1m T group were significantly higher than those in the control group(P<0.05), and there was no significant difference between the other groups compared with the control group. Lumbar vertebral compression test results show that there is no significant difference between the experimental group and the control group compared with the control group.2. Bone histomorphometric analysis results showed that the thickness of trabecular bone in 0.1mt group was significantly higher than that of the control group(P < 0.05), trabecular separation degree was significantly lower than that of control group(P < 0.05) and bone tissue area percentage was significantly higher than that of control group(P < 0.05), fluorescent double labeling distance was significantly wider than that in the control group, other groups and the control group no significant difference. CT scan analysis results and bone histomorphometry results showed the same trend.3. Analysis of serum biochemical indicators of the 0.1mt serum bone ALP(BALP) and leptin(leptin, LEP) content was significantly higher than that of the control group(P < 0.05), and tartrate resistant acid phosphatase(Tracp5b) and type I collagen C-terminal peptide(CTX-I) content was significantly lower than that in control group(P < 0.05), and other groups of serum bone formation and bone resorption markers were not significant difference with the control group.4. In the course of the experiment group 6 rats growth trends are almost consistent, control the organ index of rats in each group of liver, kidney, adrenal gland, spleen and uterus group compared with no significant difference, pathology observation, and no abnormal phenomenon. There was no significant difference between the experimental group and the control group.Integrated the results visible in the different intensity of 50 Hz TEMFs on experimental rat BMD and bone quality influence is different, in the range of 0.1 to 2.5m T only 0.1mt can be significantly improved growth rat BMD and biomechanical properties, and improve the microstructure of bone. The results of blood biochemical indicators showed that the mechanism of action is to inhibit bone resorption and promote bone formation. This paper first demonstrated that the 0.1m TTEMFs BMD was used to deal with the growth of 90 min rats, which could significantly improve the 50 Hz and bone quality of the rats, while the other organs did not produce any toxic side effects. The results of this study have important guiding significance for the application of low frequency electromagnetic field in the treatment of osteoporosis. |
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