The Relationship Between Invasiveness, Recurrence And Expression Of Ki-67, PCNA And MMP-9 In Human Pituitary Adenomas

Objective:In our study,we observe the expression of the Ki-67,PCNA antigen and MMP-9 in pituitary adenoma tissues.We observe the expression of theKi-67,PCNA antigen and MMP-9 in invasive pituitary adenoma and noninvasive pituitary adenoma,we compare the expression difference of Ki-67,PCNA antigen and MMP-9 between two groups, analysis the relationship with the invasion of pituitary adenomas.Methods:1.86 pituitary adenomas specimens removed in our department were collected, all fixed with 10%neutral formalin and then paraffin embedded.Every case history was detailedly reviewed,and all specimens were divided into invasive group(42 cases)and noninvasive group(44 cases)by adopting Hardy-Wilson and Knosp standard.2.It was examined by immunohistochemistry of SP methods that expression of Ki-67,PCNA antigen and MMP-9 in these slices of specimens.3.Finally we discuss the correlation between expression of Gal-3 and Ki-67 antigen,and then analyze the relationship between expression of Ki-67,PCNA antigen and MMP-9 with invasiveness of the pituitary adenomas.Results:1.The expression of Ki-67 and PCNA is no significant relationship with the sex in 86 pituitary adenoma,There is significant difference with diameter of the tumor,the expression of macroadenoma is higher than the small adenoma.The positive expression fig of Ki-67L1 in non-invasive pituitary adenomas andinvasive pituitary adenomas is 1.83±0.85,3.36±1.17 respectively.The positive expression rate of PCNA in non-invasive pituitary adenomas and invasive pituitary adenomas is 22.73%(10/44),57.14%(24/42) respectively.There is significant difference between 2 groups(p＜0.01).2.The expression of MMP-9 is no significant relationship with the sex in 86 pituitary adenoma,There is significant difference with diameter of the tumor,the expression of macroadenoma is higher to the small adenoma.The positive expression rate of MMP-9 in non-invasive pituitary adenomas and invasive pituitary adenomas is 47.73%(21/44),73.81%(31/42)respectively.There is significant difference between 2 groups(p＜0.01).3.The positive expression rate of PCNA in recur-invasive pituitary adenomas,non recur-invasive pituitary adenomas and afterwards recur-invasive pituitary adenomas is 55.56%, 57.58%,66.67%respectively.There is no significant difference between 3 groups(p＞0.05). The positive expression fig of Ki-67LI is 3.52±1.23,3.58±0.67 respectively.There is significant difference between 2 groups(p＜0.01).The positive expression rate of MMP-9 is 88.89%,100%,69.70%respectively.There is significant difference between 3 groups(p＜0.01).4.There is a significant correlation between expression level of Ki-67,PCNA and MMP-9.Conclusions:1.The expression of Ki-67 and PCNA in invasive pituitary adenomas is higher than that in non-invasive pituitary adenomas,the expression quantity is increased with invasion extent,indicate the Ki-67 and PCNA might be parameter in judgement invasion of pituitary adenoma.2.The value 3%of Ki-67 may be threshold value to distinguish non-invasive pituitary adenomas andinvasive pituitary adenomas,the value is higher,indicate that andinvasive pituitary adenomas is easy recurrence.3.The expression of MMP-9 is higher in invasive pituitary adenoma to non-invasive pituitary adenomas,The expression of MMP-9 is higher in recur pituitary adenoma to non recur pituitary adenoma.MMP-9 plays an important role in invasive growth and recur of pituitary adenoma.4.There is a significant correlation between the expression of Ki-67,PCNA and MMP-9 in pituitary ademona,plays an cooperative effect invasive growth and recur of pituitary adenoma,the Ki-67,PCNA and MMP-9 might be parameter in judgement invasion and prognosis of pituitary adenoma.