Xiaoyaosan Of Melanocytes Synthesis And Serum E₂ Chloasma Animal Model, The Level Of T

Objective To detect chloasma animal model before and after the administration and the healthy control group of serum estradiol and testosterone levels, while more of mushroom tyrosinase Pakistan in vitro oxidation rate of Xiaoyaosan on tyrosinase activity and to observe the impact of Synthesis of melanocytes the impact of chloasma Xiaoyaosan seeks to clarify the mechanism of control on the etiology and patogenesis Chloasma mechanism. Methods progesterone injection intramuscular injection in mice hind legs, legs turn to produce a kind Chloasma pathological changes, then to the treatment of vitamin C and Xiaoyaosan, optical microscopy local pathological changes of serum hormone (E2, T), in vitro determination of the activity of tyrosine Xiaoyaosan to observe the impact of the melanocytes synthesis of all data using SPSS statistical software package, analysis of variance and t-test.Results mice epidermal keratinocyte cells of the basal layer, there are a fairly large number of folders melanin granules positive staining of melanoma cells. Coloring deep, and sustained black, pathological lesions significantly changed, there were significant differences (P <0.05) compared with control group, model group, Xiaoyaosan large, medium and small dose group and vitamin C group or E2 , T lower, the difference was significant (P <0.05) and vitamin C group, E2: Xiaoyaosan large dose group decreased significantly, there were significant differences (P <0.05), Xiaoyaosan, no small dose group difference (P> 0.05); T: Xiaoyaosan large, medium dose group increased significantly, there were significant differences (P <0.05), low dose group there was no significant difference (P> 0.05); Xiaoyaosan large, medium and small dose E2 and T-group level changes, significant shape differences (P <0.05).Conclusion1 Xiaoyaosan histopathological lesions of chloasma to significantly improve the role. 2 Chloasma animal model of estradiol (E2) levels increased, testosterone (T) levels decreased by Xiaoyaosan and vitamin C therapy estradiol (E2) levels decreased testosterone (T) levels increased, Chloasma condition to improve its efficacy is superior to high-dose vitamin C.3 Xiaoyaosan tyrosinase activity can be increased, decreased synthesis of melanocytes, Xiaoyaosan endocrine regulation to reduce the melanocytes synthesis may be one of the mechanisms for Melasma.