The Experimental Study Of Basic Fibroblast Growth Factor In The Tendon Allograft Transplantation

Objective:To explore the effect of the exogenous basic fibroblast growth factor(bFGF) in the tendon allograft transplantation role.Methods:we optionally select 36 rats with its bilateral tendon as donor in low-temperature frozen reserve for 10 days for transplantation in 108 Wister rats whose weighing is between 220 grams to 270 grams and either sex.The others about for the 72 rats which were as receptor were randomly divided into 3 groups,and each group is 24,all of the rats were cuted one side tendon,and the frozen tendon were bridged into the defect tendon. Group A(control group):the transplanted tendon stump on both sides did not have any reagents in tendon allograft transplantation;Group B(negative control group):the transplanted tendon stump on both sides were applicated the fibrin sealant(fibrin sealant, FS) for 3μl every rat in tendon allograft transplantation;C(experimental group):tendon allograft transplantation,the transplanted tendon stump on both sides were applicated the mixture of the the bFGF and FS for 3μ1(including bFGF 500ng) every rat in tendon allograft transplantation.we selected the transplanted tendon of 12 rats in each of group to the generally,histological and biomechanical observation after 4 and 8 weeks.Results:general observation:how would be the tendon adhesion of each group after 4 and 8 weeks,the differences between A and B did not have statistically significant(P＞0.05), but between C and A,B groups,the differences did have statistical significance(P＜0.05); the group of A and B had no difference,but the group of C were better than the group of A and B after 4 and 8 weeks in the healing tendon.Histological observation:the fibroblasts and tendon tendon beam in the group of A and B was no significant difference,but between the group of C and the group of A and B were significant differences.Biomechanical Testing:the largest anti-Rally was 23.21±1.98,24.59±2.32,33.69±2.71N,and the maximum tensile displacement was 4.52±0.43,4.38±0.27,3.01±0.35 mm in the group of A,B and C after 4 weeks,there were no statistically significant between A and B groups (P＞0.05),but there were significally statistics between the group of C and the groups of A and B in every indicators(P＜0.05);the largest anti-Rally was 40.82±3.66,41.62±3.49,50.34±4.81N,and the maximum tensile displacement was 2.91±0.25,2.85±0.36,1.83±0.17mm in the group of A,B and C after 8 weeks,there were no statistically significant between A and B groups(P＞0.05),but there were significally statistics between the group of C and the groups of A and B in every indicators(P＜0.05).Conclusion:(1) The exogenous bFGF can promote the healing speed of the tendon transplant in transplantation tendon stump of the rates,but also increase the tendon adhesion formation(2) Cryopreservation tendon can reduce tendon antigenicity.