Expression And Significance Of HMGB1,PCNA In Leiomyoma Of Uterus

Objective: To determine the expression of high mobility group box 1(HMGB 1) and proliferating cell nuclear antigen (PCNA) in the uterine leiomyoma and the control groups ,and to investigate the role of HMGB1 in the pathogenesis of uterine leiomyoma .Methods: Immunohistochemical method was employed to detect the expression of HMGB1 in the uterine leiomyoma and the other groups, the same methods to detect the expression of PCNA as a control. Result: 1.There were some certain extent expression of HMGB1 and PCNA in the uterine leiomyoma and the control group .Compared with the relative normal group (normal myometrium by side of leiomyoma), the positive expression of HMGB1 and PCNA in the uterine leiomyoma were significantly higher (p<0.05). 2.There was no significant difference for positive expression of HMGB1 between single leiomyoma and multiple leiomyoma,( p>0.05), the same as the expression of PCNA . There was no significant difference for positive expression of HMGB1 between the small leiomyoma and the big one, (p>0.05) .the same as the PCNA. 3.The expression of HMGB1 and PCNA was positive correlation,(rs =1 , p>0.05).Conclusion: 1. Both HMGB1 and PCNA are expressed in every group suggests that: their existence is widespread. 2. The high expression of HMGB1 in uterine leiomyoma indicates HMGB1 is related with the development of the uterine leiomyoma. 3. The positive correlation between HMGB1 and PCNA suggest that HMGB1 is related with cell proliferation. And this relationship maybe one of the etiology of the uterine leiomyoma . 4. The positive expression of HMGB1 was mostly located and in the cell karyon in uterine leiomyoma. This character maybe direct new methods to prevent, diagnose, treat uterine leiomyoma.