Effect Of Diallyl Disulfide On The Proliferation Of The Human Leukemia HL-60 Cell Line And Its Mechanism Approach

Objective To investigate the proliferation of human leukemic HL-60 cell line and the correlation between the expreesion of survivin protein and cell cycle treated by diallyl disulfide(DADS).Method MTT was used to test the growth of HL - 60 cells treated by DADS;SP-immunohistochemical and flow cytometry were used to study the expression of survivin protein in HL-60 cells and cell cycle arrest treated by DADS.Results DADS significantly inhibited the proliferation of HL - 60 cells and the inhibiting effects had a dose -time dependence(r＞0.9, p＜0.01).The inhibition fraction of HL - 60 cells rose from 8.94%to 70.66%with DADS increasing from 0.625mg/L for 24 hours to 20.0mg/ L for 72 hours.Survivin was expressed in blank control group and DMSO solvent group at very high level.Their SⅡare 1125 and 1126 respectively and the difference between them is not statistically significant(P＞0.05).The expression of survivin protein could be down regulated by 5,10,and 20mg/L DADS-treated HL-60 cells for 24,48 and 72 hours and the effects had a time-dose dependence(r＞0.9,p＜0.01).The SⅡof HL-60 cells treated by 20mg/L DADS for 72 hours is 119,which is the lowest level in the test.The differences in the DADS -treated HL-60 cells groups and the control group were all statistically significant (P＜0.01),there were also statistically significant differences among the three DADS -- treated HL-60 cells groups(P＜0.01).G2/M phase arrest witch is accompanied with the down-regulated of survivin protein could be found in DADS interference in a dose-time dependent manner (r＞0.9,P＜0.01)Treated with 20mg/L DADS for 72 hours,the percentage of cells in G2/M phase rose to the highest level of 66.56%.Conclusion DADS can effectively inhibit the proliferation of HL-60 cells,down-regulate the level of survivin protein and bring about G2/M phase arrest.