Study On Immunological Regulation Of Different Dose Arginine In Hepatic Failure Rats

ObjectiveTo observe the effects of different dose arginine on liver-renal function, nutritional status and immune function in hepatic failure rats induced by the D-Galactosamine Hydrochloride, and explore the mechanism further to provide experimental data and rationale for remedy clinical hepatic failure patients.Methods1. The AHF model in rats10 healthy male wistar rats were randomly divided into normal control (NC) and hepatic failure control (HFC) groups. The rats in HFC group were induced by the D-Galactosamine Hydrochloride. After abrosia 12 hours, the rats, were injected 10% D-galactosamine 1.2g/kg into abdominal cavity, to cause the model of acute hepatic failure, the NC were injected normal sodium. The liver-renal function, plasma protein, glyc- lipid metabolism and the pathological change of liver were observed after 24 hours. Another 6 were observed general objective sign and survival.2.The study on immunological regulation of different, dose arginine in AHF rats60 healthy male wistar rats were randomly divided into six groups: A (NC), B(HFC), C(ArgⅠ,0.4 g·kg^-1·d^-1),D(ArgⅡ,0.8 g·kg^-1·d^-1), E(ArgⅢ, 1.6g·kg^-1·d^-1), F(ArgⅣ, 3.2g·kg^-1·d^-1). The glycine was used to make up for nitrogen for other hepatic failure groups. The nutritional therapy was started after 24 hours. NCgroup was gived common animal feeds. The liver-renal function, clotting mechanism, plasma protein, glyc- lipid metabolism, immune function and the pathological change of liver were observed at 14 d.Results1. The AHF model in ratsAfter injection of D-Galactosamine Hydrochloride, the rats of model group appeared activity reduce, food-intake descend, lethargy, dull or excessus of reaction, crazy, twitch, deep yellow urine,bleeding from mouth ,eye and stool, the capill of all over the body was moist and none gloss. 3 were died in 6 rats between 48h and 72h. The indexof biochemistry detection in model group were significantly difference than those in NC group (P<0.05). The liver was found obvious pathological change in model group.2. The study on immunological regulation of different dose arginine in AHF rats(1) The effects of arginine on death rate,weight and food-intake in AHF rats: Arginine maked the death rate lower than HFC group, but the difference there was no statistical significance in six groups.The weight and food-intake were significantly lower than those in group A (P<0.05). The recover of weight and food-intake after 3 days, until the end of experiment (P<0.05).(2) The effect of arginine on nitrogen balance and plasma protein in AHF rats: Hepatic failure rats were negative nitrogen balance until 10 days. The serum contents of TP, ALB, PA, RBP in group D and group E were significantly higher than those in group B (P<0.05).(3) The effect of arginine on liver-renal function AHF rats: The serum contents of ALT,AST,ALP,TBIL,BUN,Cr,GLU,TG,CHLO,MDA in Arg groups were significantly better than those in group B (P<0.05). In four Arg groups, liver-renal function in group D and group E were better than those in group C and group F.(4) The effect of arginine on immune function AHF rats: The serum contents of IgA,IgM,IgG,IL-2,IL-10,IGF-1 in groupB, group C, group D, group E, group F were lower than those in group A. While the TNF-a in group B and group C were higher than that in group A (P<0.05). The IgA and IgM in in group D and group E were higher than those in group B (P<0.05).The IL-2 in group D, group E, group F were higher than those in group B (P<0.05).The NO in group F were higher than those in group A and group B (P<0.05).(5) The pathological change of liver in group D and group E was better than that in groupB, group C, group F,inflammatory reaction was slighter than other groups.Conclusion1. D-Galactosamine Hydrochloride could be used in copying the acute hepatic failure model in rats. General objective, death rate, biochemistry detection, the pathological change of liver were obvious. The rats could reach the level model success.2. Arginine could relieve the physical stringent state of AHF rats ,enhancing the function of immune, improving protein metabolism, promoting the retrieve of nutritional status. Adoptive four diffenent dose manifested dose-effect relationship, 1.6 g·kg^-1·d^-1 was better than low and high dose. At secure and curative dose, arginine was in favour of the retrieve of liver cell metabolism and clotting mechanism, improving liver enzyme system and function, enhancing the function of immune and synthesis of protein.