The Therapeutic Effects Of Decapeptide CMS001 On Renal Anemia And Its Mechanisms

Objective:To investigate the therapeutic effect of decapeptide CMS001 on Renal Anemia and its possible mechanisms.Methods:1.Two kinds of animal model were used to investigate the therapeutic effect of decapeptide CMS001 on Renal Anemia.The one was adenine of intragastric administration to the Wistar rats,and the other was 5/6 nephrectomy on rats.The therapeutical effect of CMS001(80μg/kg/d,40μg/kg/d,20μg/kg/d)on peripheral hemogram and renal function of renal anemia rats were observed.2.HE stain was used to observe the therapeutical effect of CMS001 on pathological damage of kidney of RA rats.3.The effect of CMS001 on EPO protein level in the kidney of RA rats was assayed by Western Blot methods.4.The effect of CMS001 on EPO mRNA level in the kidney of RA rats was assayed by Real-time PCR methods.Results:1.At the dose of 80μg/kg/d,40μg/kg/d,CMS001 can significantly increase sum total of erythrocyte,hemoglobin and hematocrit in the peripheral blood of RA rats (P＜0.05).CMS001 also can significantly decrease serum BUN and Scr of RA rats (P＜0.05).2.At the dose of 80μg/kg/d,40μg/kg/d,CMS001 can ameliorate the kidney pathological damage of RA rats.3.At the dose of 80μg/kg/d,40μg/kg/d,CMS001 can remarkably increase EPO protein expression level in the kidney of RA rats(P＜0.05).4.At the dose of 80μg/kg/d,40μg/kg/d,CMS001 can remarkably increase EPO mRNA level in the kidney of RA rats(P＜0.05). Conclusions:Decapeptide CMS001 has the therapeutic effect on RA rats.CMS001 can remarkably increase protein expression level and EPO mRNA level in the kidney of RA rats.CMS001 can significantly increase sum total of erythrocyte,hemoglobin and hematocrit in the peripheral blood and decrease serum BUN and Scr of RA rats. CMS001 can ameliorate the kidney pathological damage of RA rats.