| Objective: Vascular Dementia (called VD as follows) is one of the important types in senile disease. With the aging of the population, the incidence of VD is more and more higher; VD has become an important factor to affect seriously the people's living quality. Therefore, it's of great significance to make deeper research into its pathological mechanism and develop the effective treatment to prevent and cure the VD. Based on the conventional knowledge and the modern research achievements of Traditional Chinese medicine (TCM) on VD, my teacher developed QiangLiZengZhiLing with the function of promoting blood circulation to remove meridian obstruction, dissipating phlegm to descending the turbid, benefiting kidney and brain. In order to make probe into its treatment action mechanism, we adopted VD model of Kunming mice with the operation of ligating both side carotid artery, observed its effect on mice praxiology, hippocampus pathomorphology, the contents of SOD, MDA in brain tissue neurotransmitter, etc.Method: We duplicated the model of VD mice with the operation of ligating both side carotid arteries. 100 healthy male Kunming mice were divided into five groups with 20 mice in each group at random,which were sham-operation group, model group, high dose of QiangLiZengZhiLing group(called"High-dose group"as follows), low dose of QiangLiZengZhiLing group (called"Low-dose group"as follows),and Nimodipine control group (called"Nimodipine group"as follows). We duplicated the model of VD mice with the operation of cerebral ischem reperfusion through separating and ligating both side carotid arteries with putting blood from coda except sham-operation group. And then 5 groups were continuously filled medicine or saline through the stomach from the second day after the operation of cerebral ischem reperfusion. Sham-operation group and model group were filled saline and other groups were given medicine. We did the praxiology experiment On the 7th and 15th day after operation (jumping stand experiment, water-labyrinth experi- ment). The mice were cut head for obtaining cerebral tissue, the left part of which was fixed and cut into slices. The tissue was stained by HE (Hematoxylin) and Nissl dye. We observed constitutional morphologic changes of cerebral tissue under light microscope. Then we made the homogenate from cerebral cortex tissue, took the supernatant after centrifuging, measured the content of the SOD, MDA in the brain tissue with colorimetric analysis antigenic and examined the change of the brain neurotransmitter (NE and 5-HT) with high-performance liquid chromatography.Results1 The effect of QiangLiZengZhiLing on praxiology of VD mice. 1.1 The mice's performance record of jumping standEvery period after the operation, model group had longer reaction time, shorter latency time and more wrong times, there was different (P<0.01) compared with Sham-operation group. At the same time, other groups had shorter reaction time, longer latency time and less wrong times, there was different ( P<0.05 or P<0.01) compared with model group.1.2 The mice's performance record of water-labyrinthEvery period after the operation, there was no significant difference between the learning and memorizing performance. The wrong times and the time of swimming along the whole line of the model group were more than those of the sham-operation group (P<0.05 or P<0.01). There was different (P<0.01) compared with the Sham-operation group. At the same time,the swimming time and the wrong times of other groups were less than those of the model group (P<0.05 or P<0.01), and the performance of the High-dose group was better than that of the Low-dose group. There was no significant different between the Little-dose group and the Nimodipine group.2 The effect of QiangLiZengZhiLing on cerebral pathomorph- ology of VD miceThe pathomorphology under light mirror lens indicated that pyramidal cells in the hippocampus of the sham-operation group were arranged closely, cell nucleuses were big and round, nucleoli were clear. The layers of the pyramidal cells in the hippocampus of the model group were reduced, the pyramidal cells were arranged sparse and missed obviously, the cell nucleuses diminished and the cell structure was vague The scope of the pathological changes in hippocampus of mice brain tissue involved CA1, CA2 and CA3 areas with CA1 area was especially obvious, hippocampus of mice brain tissue took on the pathological changes of ischem. As time went, the pathological changes of brain tissue in the model group was growing seriously. The pathological changes of each medical group were improved compared with the model group.3 The effect of QiangLiZengZhiLing on the content of the SOD, MDA in the brain tissue of the VD mice.The activity of the SOD in the brain tissue of the model group was lessoned compared with that of the sham-operation group (P<0.01), but the content of the MDA was increased obviously (P<0.01); The activity of the SOD in the brain tissue of the medical group was strengthened compared with that of the model group (P<0.01), but the content of the MDA was decreased obviously (P<0.01). The activity of the SOD in the brain tissue of high-dose group was higher than that of the low-dose group and the Nimodipine group (P<0.05), but the content of the MDA was decreased obviously (P<0.05). There was no different when other medical groups were compared with each other (P>0.05).4 The effect of QiangLiZengZhiLing on the content of 5-HT and NE in the hippocampus brain tissue of the VD mice. The content of the NE, 5-HT in the hippocampus brain tissue of the model group were lower obviously than those of the sham-operation group (P<0.01 or P<0.05); The content of the Ne and 5-HT in the hippocampus brain tissue of the medical groups were higher obviously than those of the model group (P<0.01 or P<0.05); The content of the NE in the hippocampus brain tissue of high-dose group was higher than that of the Nimodipine group (P<0.05); we could draw the conclusion that there was different, but there was no statistics significance in the difference between the low-dose group and the Nimodipine group (P>0.05).Conclusion1 The descend of the study and memory ability resulted from the cerebral ischem is the important reason to cause VD, QiangLiZengZhiLing can raise the VD mice's performance record of water-labyrinth and jumping stand experiment, and improve the study and memory ability.2 The damage of the pyramidal cells in the hippocampus tissue is the import pathology change that will lead to VD, QiangLiZengZhiLing have treatment function to the VD mice, and can alleviate the pathology damage caused by the cerebral ischem.3 QiangLiZengZhiLing can raise the activity of the SOD, lower the content of the MDA to resist the damage of the free radical. 4 QiangLiZengZhiLing can heighten the content of the NE and 5-HT in the the hippocampus tissue of VD mice, and allevite the damage of the cerebral ischem.
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