Clinical Significance Of E-cadherin/Catenin Re-expression In Metastatic Lymph Nodes And The Expression Of NF-κB And Rb Protein From Advanced Gastric Carcinoma

Objective: The aim of this study was to study the expression of NF-κB, Rb and the re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes from advanced gastric carcinoma,and to evaluate its clinical significance,to investigate the unite factor effect of NF-κB, Rb and E-cadherin/catenin complex for metastatic mechanism of gastric carcinoma and prognosis. Therefore , to look for a kind of more sensitive and reliable method for early period diagnosis and early period remedy in a molecule level of gastric carcinoma.Methods: S-P immunohistochemical technique was used to examine the expression of NF-κB, Rb in primary advanced gastric carcinoma and the re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes from 86 advanced gastric carcinoma. The level of the expression was analyzed combined with clinicopathological features of the size of the tumor, infiltration depth, pathological differentiation, tumor pathological stages, lymph node metastasis and prognosis. Results: The color of the immune positive products of NF-κB,Rb was buffy and located in cytoplasm or nucleus and E-cadherin/catenin complex was buffy and located in plasmalemma. The rate of positive expression of NF-κB was 81.40% (70/86) in primary advanced gastric carcinoma. The rate of positive expression of Rb was 65.12% (56/86) in primary advanced gastric carcinoma. The rate of positive re-expression of E-cadherin/catenin complex was 63.95% (55/86) in cancerous cells of metastatic lymph nodes from advanced gastric carcinoma. The expression of NF-κB was lower in mucinous adenocarcinoma than those in adencarcinoma and the rate of positive expression was 61.90% (13/21) and 85.96% (49/57) respectively (P < 0.05). The expression of NF-κB was lower in poor differentiation degree than those in high and moderate differentiation degree and the rate of positive expression was 74.51% (38/51) and 91.43% (32/35) respectively (P < 0.05). The expression of NF-κB was not remarkable difference in the diameter in the primary advanced gastric carcinoma and the rate of positive expression was 83.08% (54/65) and 76.19% (16/21) respectively (P > 0.05). The expression of NF-κB was not remarkable difference in the infiltrated range limited to muscle stratum than serosa stratum and the rate of positive expression was 82.05% (64/78) and 75.00% (6/8) respectively (P > 0.05). The expression of Rb was lower in poor differentiation degree than those in high and moderate differentiation degree and the rate of positive expression was 74.51% (38/51) and 85.71% (30/35) respectively (P < 0.05). The expression of Rb was not remarkable difference in the mucinous adenocarcinoma than those in adencarcinoma and the rate of positive expression was 70.18% (40/57) and 71.43% (15/21) respectively (P > 0.05). The expression of Rb was not remarkable difference in the diameter in the primary advanced gastric carcinoma and the rate of positive expression was 60.00% (39/65) and 80.95% (17/21) respectively (P > 0.05). The expression of Rb was not remarkable difference in the infiltrated range limited to muscle stratum than serosa stratum and the rate of positive expression was 74.36% (50/78) and 75.00% (6/8) respectively (P > 0.05). The re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes was lower in poor differentiation degree than those in high and moderate differentiation degree and the rate of positive expression was 54.90% (28/51) and 77.14% (27/35) respectively (P < 0.05). The re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes was not remarkable difference in the mucinous adenocarcinoma than those in adencarcinoma and the rate of positive expression was 52.38% (11/21) and 73.68% (42/57) respectively (P > 0.05). The re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes was not remarkable difference in the diameter in the primary advanced gastric carcinoma and the rate of positive expression was 64.62% (42/65) and 61.90% (13/21) respectively (P > 0.05). The re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes was not remarkable difference in the infiltrated range limited to muscle stratum than serosa stratum and the rate of positive expression was 74.36% (50/78) and 62.50% (5/8) respectively. There is no remarkable difference between the groups by Wilcoxon's log-rank test. And the Cox's proportional-hazard show that the re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes was a independent factor of prognosis. The expression of NF-κB, Rb and the re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes was not significant correlated among each (P > 0.05).Conclusion: NF-κB, Rb and E-cadherin/catenin complex may be closely correlated to the occurrence, the advancement and the metastasis of gastric carcinoma. The expression of NF-κB, Rb and the re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes was increased from advanced gastric carcinoma. The re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes was closely correlated and could be a independent factor of prognosis. The expression of NF-κB, Rb was not correlated to the prognosis. The expression of NF-κB, Rb and the re-expression of E-cadherin/catenin complex in cancerous cells of metastatic lymph nodes was not significant correlated among each. Therefore, NF-κB, Rb and E-cadherin/catenin complex could be checked to enhance the sensitivity of diagnosis and remedy early period and to improve the prognosis.