| The Bio-effect of impulse MF (Magnetic field) on tumor cell both in vitro and in vivo has been becoming one of the research focuses since many years. Although the fact is that there are many papers published on this field of MF bio-effect, the most frequent method that is used to do this research is the combination of statistics and epidemiology investigation, especially to the study of MF. On this paper, MF will be employed to act on the tumor cell, in vitro and vivo with the same frequency and different intensity (0.5mT,1.0mT and 1.5mT),Moreover, modern biotechnology and method will be used to clarify the effect of different intensity MF to the cell's growth,proliferation and differentiation.Part One The study of MF induced K562 cell'growth,proliferation and differentiation and cell cycleObjection: To clarify the mechanism and principle of LMF acted on tumor cell through the research of LMF influence on cell's growth,proliferation and differentiation; Hopefully, it could provide some theory base and references on the clinic therapy of human tumor.Method: The cultured K562 cells which have been treated with 3 groups of different intensity MF, take use of the colorimetric 5-diphenyl tetrazolium bromide (MTT) to investigate the impact on the proliferation and differentiation of tumor cells. Besides, the corresponding relation between the LMF and the tumor cell cycle will be determined with Flow cytometry; the morphological changes on K562 cells induced by LMF can be observed in the presence of light,electron microscopy and photo analysis,Results:1. The results show that the proliferation amount of K562 cells, compared to pre-treated only one group (1.0mT) indicated that it is significant on statistics. The longer the cells of this group exposure on the MF; the stronger the inhabitation of this group cells become, There is no any significance on the other two groups. It is easy to conclude that MF is able to restrain the growth of tumor cells only under special intensive range.2.The poison phenomenon will be emerging after 6 hours on this group, 1.0mT, the cells became bigger and bigger. the volume,diameter and ratio of nucleus/cytoplasm of K562 cells are decreased, while the heterochromatin are condensed in nucleus after 24 hours. It differentiates toward mature direction. It is very normal on the group of 1.5mT, even after 72 hours.3. The cell amount of from G0 to G1 will go up while it will drop G2/M if the usage dose of MF increased. It is quite obvious on this group of 1.0mT. The occurrence ratio of apoptosis will sum up. Meanwhile, the results showed that different intensity LMF primarily affect the procedure from G2 to M in the process of cell cycle.Part Two Research of combined MF and HIFU tumor therapy. Objection: Explore the effect that LMF was applied to destroy VX2 tumor cell lines of New Zealand rabbit, and the impact of combined LMF and HIFU with the treatment of tumor.Methods: 50 New Zealand Rabbits were divided by 2 groups, one is the control, and another is the treatment group. The option of all MF parameters is based on the results of Part One, namely 1.0mT and Frequency 30Hz were selected as the treatment parameters.VX2 cell line were transplanted into the liver of rabbits in terms of activation and culture cells, and build up the tumor model.Control and Treatment will continue to be dealt by HIFU after they got MF done. The parameters of HIFU as follows: Frequency of treatment head is 1.6MHz, the intensity of ultrasound will reach to 2000W/cm2.All groups will get treatment under the scan of B Mode.Results1.The average diameter and volume of tumor cell of treatment group, compared with control, is far smaller. It is very obvious that the cells of control are far bigger than the treatment.2. Those cells of treatment, swell, cell plasma loosen, vast bubble filled with plasm, nucleolus,3. The average time of the treatment beyond 32 Gray Degree is about 12s, in contrast, the controls need 32s to reach the same Gray Degree. Among the controls, there are 8 rabbits that showing some skin canker. The treatment only 1 showed this skin damage.Conclusion:â‘ MF has quite apparent inhibitory effect on tumor cells within special intensive range.â‘¡MF is capable of resulting in the end of tumor cell proliferation cycle within some intensive value range.â‘¢To some extent, MF with the intensity of 1.0mT has the ability to inhibit the growth and proliferation of VX2; No formation of apoptosis under the observation of microscopy;â‘£the treatment time is shorter than the controls, and the damage to the skin is smaller.
|
PDF Full Text Request