| [OBJECTIVE]1.To investigate the difference of plasma levels of homocysteine(Hcy)between patients of major depressive disorder(MDD)and normal controls.2.To study the influence of polymorphisms of 5,10-methylene tetrahydrofolate reductase(MTHFR)on the plasma Hey levels.3.To explore the relationships of polymorphisms of MTHFR gene C677T and MDD.[METHODS]1.A total of 77 MDD cases were sampled from hospitalized patients of Shandong Mental Health Center.Moreover,a total of 85 healthy controls were taken from medical examination center of a certain general hospital.2.Basic information questionnaire was administrated to all subjects.Hamilton Rating Scale for Depression(HAMD,17-item)and Hamilton Anxiety Scale (HAMA)were examed at the baseline in the study group.3.The plasma Hey levels were determined by fluorescence polarization immunoassay(FPIA).And,the genotypes of MTHFR C677T were detected by polymerase chain reaction-restriction fragment length polymorphism(PCR -RFLP)in two groups. [RESULTS]1.To compare the plasma Hcy levelsThe plasma levels of Hcy in the study group were significantly higher than those in the control group.There was a higher incidence of hyperhomocysteinemia(HHcy) in study group than in control group.No difference of Hey levels was investigated between male and female in both study group and control group.Relapse patients had higer Hey levels than the first attack patients and the patients with positive family history had significantly higher Hey levels than those with nagitive family history.Hey levels of any genotypes in study group are higher than that of corresponding genotypes in control group.Plasma Hey levels of TT genotype in patients and controls were obviously higher than those of CT and CC genotype.2.The polymorphisms of MTHFR gene C677TThe distributions of MTHFR gene C677T polymorphism in study group and control group were all in accordance with the law of Hardy-Weinberg equilibrium (P＞0.05).There were significant differences in genotype frequencies and allele frequencies between study and control group(P＜0.05).T allele frequency in study group was higher than that in control group.For MDD,the odds ratio for TT versus non-TT genotype was 2.464,95%CI: 1.205～5.038.The distributions of MTHFR genotypes were associated with gender(P＜0.05) but no association was found with frequency of MDD attacks and family medical history.(P＞0.05)。3.The score of HAMDThe total scores of HAMD in different genotypes of MTHFR had significant differences(F=18.958,P=0.000)。Compared to CT and CC,TT genotype had higher HAMD scores(P＜0.05);There was no significant difference of HAMD scores between CT and CC(P＞0.05)。 The total scores of HAMD were positively relevant to plasma Hey levels(r= 0.473,P=0.002)。4.The score of HAMAThe total scores of HAMA in different genotypes of MTHFR had no significant differences(F=0.257,P=0.774)。There was no significant difference of HAMA scores among TT,CT and CC(P＞0.05)。The total scores of HAMA were not relevant to plasma Hey levels(r=0.112, P=0.333)。5.The main influencing factors of Hcy in MDD patientsBy adopting multiple stepwise regression analysis,the plasma Hcy levels were significantly correlated with age,TT genotype,frequency of MDD attacks and family medical history。[CONCLUSION]1.Elevation in plasma Hcy levels should be a risk factor of major depressive disorder.Plasma Hcy levels may be used to predict the severity of MDD.2.The related factors of Hcy include:age,frequency of MDD attacks,family medical history and polymorphisms of MTHFR C677T.3.The polymorphisms of MTHFR C677T may be hereditary predisposing factor of MDD,which may be included in the onset and clinical situation of MDD by influencing levels of Hcy. |
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