The Mechanism Of Cordycepin On Reducing Triglyceride In HepG₂ Cells

Objective To investigate the role and mechanism of cordycepin on reducing triglyceride in cultured HepG₂ cell line. Methods The molecular docking of cordycepin, cordycepin receptor and AMP activated protein Kinase was carried out using AutoDocK4.0 software and CSI-MS analysis. The cytotoxicity of cordycepin to HepG₂ cells was measured by MTT assay. The content of triglyceride in HepG₂ cells was determined by triglyceride kits; the AMPK activity were detected by ATP easylite-kinase kits, the expression of PPAR protein were analyzed by Western Blot. The residual content of cordycepin in HepG₂ cells after metabolism was analyzed by HPLC. Results the Molecular docking experiments of cordycepin and CSI-MS analysis showed that cordycepin could be well matching with AMPK; Compare to the control group, cordycepin has significantly increased HepG₂ cell's surviving ratio and reduced the triglyceride content in HepG₂ cell line in dose and time dependent way. It might be realized by means of AMPK activation, and inducing the PPAR alpha expression down stream because of 98% of cordycepin in HepG₂ cells had metabolisis in two hours. Conclusion cordycepin could reduced the triglyceride in HepG₂ cells significantly and its might be induced by activating AMPK and its downstream PPAR expression; The cordycepin in the cells metabolism so fast, but lipids are relatively durable, it is need to further research yet.