Association Of C-159T Polymorphism In Promoter Region Of CD14 And Diabetic Nephropathy

Objective : To investigate the distribution of CD14 promoter geneC-159T polymorphsim in T2DM patients of Yanbian population and analyze the association of CD14 polymorphisms with diabetic nephropathy (DN), study the risk factors of DN . Methods : Genotypes of CD14 were typed in 308 T2DM patients and 145 normal controls by polymerase chain reaction-restriction fragment length polymorphism ; conducting a retrospective analysis of clinical features of 308 T2DM patients(76 patients with DN). Results: (1) The distribution of CD14 promoter geneC-159T polymorphsim has no significant difference between normal control group and T2DM group(p>0.05). The distributions of CC homozygous genotype v.s. CT heterozygous and TT homozygous genotype exist statistically significant difference in CD14 C-159T between non-diabetic nephropathy subgroup and diabetic nephropathy subgroup (p<0.05). (2) At the process of 10 and 20 years, the percent of T2DM patients develop into DN is 13.3%and 23.4%. CC homozygous genotype has higher risk than the CT heterzygous and TT homozygous genotype to develop into DN (0R=1.621, 95% CI: 1.010～1.737). (3) DN has close relation with hypertension, process, CRP, fasting blood glucose and low density lipoprotein-cholesterol (p<0.05 or p<0.01) but no relation with Age, PBG, HbA₁c, Cr, CHO, TG, HDL-C and BMI(all p>0.05). Conclusion : (1) The distribution of CD14 promoter geneC-159T polymorphsim has no relation with onset of T2DM. The CC genotype of the C-159T polymorphism of CD14 gene is a risk factor for diabetic nephropaty. (2) Hypertension, long process, high fasting blood glucose, low density lipoprotein-cholesterol ascension and inflammatory reaction is important risk factors for DN.