Expression And The Clinical Significance Of Nuclear Factor-κB In Middle Ear Infective Diseases

Objective:To investigate the expression and effect of NF-κB in the middle ear cholesteatoma and the granulation tissues,and illuminate the pathogenesis of middle ear infective diseases.Method:All the cases were divided into 3 groups:25 cases of middle ear cholesteatoma, 25 cases of middle ear granulation tissues and 15 cases normal external ear canal skin specimens.The expressions of NF-κB in the cases were detected using S-P dyeing method by immunohistochemistry.Result:In the 25 cases of cholesteatoma of middle ear,NF-κB were strong positive in18 cases and moderate positive in 7 cases;all cases were positive in cytoplasm,and 10 were positive in nucleolus;the NF-κB expressions were most located in basal lamina and prickle cell layer,and some were located in inflammatory cells and vascular epithelial cells.In the 25 cases of granulation tissues,NF-κB were strong positive in8cases and moderate positive in 16cases and weakly positive in one case;the NF-κB expressions were located in inflammatory cells;except 3 cases were positive in nucleolus,all cases were positive in cytoplasm.And there were negative or weakly positive of NF-κB in the epidermis of auditory canal.The express intensity of NF-κB in the cholesteatoma epidermis is higher than that in the middle ear granulation tissues.The express intensity of NF-κB in the Granulation tissue is evidently higher than that in the external auditory meatus epidermis(P＜0.01).Conclusion:NF-κB activation plays an important role in the middle ear infectious disease's occurrence and development.The further research NF-κB that expressed in the middle ear cholesteatoma and the granulation tissue possibly provied new breakthrough for the middle ear infectious disease pathogenesis,the clinical diagnosing and treating.