| Objective This study was designed to evaluate the role of connective tissue growth factor (CTGF) in subconjunctival tissue wound healing.Experiments were performed in a rabbit model.Methods Operation was randomly performed on one eyes of 15 rabbits,the other eye was not performed the operation and was used as the control.On days 1,5 and 9 after operation they were killed in batch.Tissue was harvested from the conjunctival and subconjunctival tissue.One part of the tissue was made pathological section,then was H-E stained and immunostained and observed under light microscope.The other part of the tissue was detected by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR) analysis.Results1.Histological examination showed that fibroblasts,inflammatory cells,collagen and vasodilatation occurred in the early stage after surgery.5 days after surgery, fibroblasts,inflammatory cells and collagen increased.9 days after surgery,fibroblasts, inflammatory cells and diminished,while collagen increased and densed,and neogenetic blood vessels appeared.2.Immunohistochemistry staining revealed that the staining of CTGF was enhanced 1 day after surgery.5 days after surgery,the staining of CTGF was enhanced obviously.9 days after surgery,the staining of CTGF weakened but still more obvious than normal.3.RT-PCR results:CTGF mRNA was up-regulated 1 day after surgery and came to the culmination 5 days after surgery.9 days after surgery,CTGF mRNA began to descend,but it was still higher than the contrast group,and the difference was statistically significant(p＜0.01).Conclusion In the progress of rabbit subconjunctival tissue wound healing,the expression of CTGF increased at both protein and mRNA levels.It suggests that inhibition of CTGF synthesis or its action may help prevent bleb failure and improve glaucoma filtration surgery outcome. |
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