Clinical Investigations On The Connective Tissue Growth Factor In Aqueous Humor Of Glaucomatous Eyes

Purpose:To assess the CTGF in the aqueous humor of glaucoma eyes,comparing with those of eyes with cataract only,used as control,in order to assess the role of CTGF in the pathophysiology of glaucoma.Methods:0.05-0.1ml of aqueous humor were collected from 54 primary glaucoma eyes and 24 eyes with simple cataract,which were used as control.The concentration of CTGF were measured with double antibody Sandwich engyme-linked immunosorbent assay(ELISA).In view of the normal distribution with 1-Sample K-S Test,the CTGF concentrations in patient groups were compared,and the statistical significance of the results was analyzed with One-Way ANOVA.Results:The concentration of CTGF in eyes with simple cataract(n=24)was 1.26±0.05mg/ml (mean±standard deviation),which was significantly lower(P＜0.0001)than that in eyes with primary acute angle-closure glaucoma(n=34,2.19±0.06ng/ml)or primary chronic angle-closure glaucoma(n=12,1.71±0.05ng/ml)or primary open angle glaucoma(n=8,2.19±0.06ng/ml).In addition,eyes with acute angle-closure glaucoma had significantly higher CTGF concentrations than eyes with chronic angle-closure glaucoma or primary open angle glaucoma(P＜0.0001), whereas significant difference was found between groups of chronic angle-closure glaucoma and open angle glaucoma(P＜0.0001).There are no significant difference by sex within groups analyzed with t test.Conclusion:①The concentration of CTGF in glaucoma patients' aqueous humor was significantly higher than that in cataract patients' aqueous humour.②The concentration of CTGF in eyes of male was no significant than that in eyes of female.