| In this study, nanoparticles encapsulated with or without insulin were prepared via the self-assembly in aqueous medium with polyethylene glycol-graft-poly (lactic acid) (PPLA) as raw material. Their hemolysis properties were investigated. The solubility ofPPLA in water was (2.16～4.32)×10-2 mg·mL-1 according to eye judgment. The critical micelle concentration was 1.12×10-3 mg·mL-1 obtained from fluorospectrophotometer. The self-assembled aggregates of PPLA in water were nanoscale spheres observed by transmission electron microscope. The particle size and zeta potential tests by dynamic light scattering revealed that during preparation, the ratio of hydrophi-licity/hydrophob-icity, the aqueous medium and the pH had great influences; while dilution, freezing and pH change to basic condition had no effect after preparation, but pH change to acidic condition precipitated nanopaticles. The results indicated the particle size and zeta potential of PPLA nanoparticles could be easily controlled via the changes of condition in or after preparation. The anti-non-specific protein adsorption property of PPLA nanoparticles was confirmed by the FIY+TC-BSA absorption tests compared with particles of PLA.With the nanoparticles of various concentration and particle size, the % hemolysis were investigated. The results demonstrated that the particles size had great influences in the % hemolysis besides the concentration. The critical hemolysis concentration was 1.5～2.0 mg·mL-1, at which the % hemolysis was just above 5%.Insulin loaded PPLA nanoparticles were prepared by the self-assembly in aqueous medium. They got good loading rate and controlled release results. The % hemolysis of insulin loaded PPLA nanoparticles were lower that the plain PPLA nanoparticles prepared in the same condition and than the insulin with the same concentration resulted from 100% release of insulin-loaded nanoparticles.All of the results suggested the postential applications of PPLA in drug delivery.
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