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The Experimental Research About Antipyretic And Analgesic Effects Of Anti-fever 2 And Its Possible Antipyretic Mechanism In Central Neural System

Posted on:2005-07-02Degree:MasterType:Thesis
Country:ChinaCandidate:P HuangFull Text:PDF
GTID:2144360122491043Subject:Physiology
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The Chinese traditional medicine is our precious treasure. Recently more and more new herbs have been impoldered. The anti-fever 2 whose main components are Puerariae Eordythia Andrographis Paniculata and Agastache is just a new compound agent. Before reporting it to a higher authority, we take physiological and pharmacological methods to observe its antipyretic analgesic anti-inflammatory in vitro bacterisostatic effects. This paper mainly talk over the antipyretic and analgesic effects of anti-fever 2 on laboratory animals and how it brings into full play. Our purpose is to introduce experimental thereunder and theoretic base for clinical use and further usage.Materials1. Animal; male Wistar rats^KunMing mice2. Drug: anti-fever 2 powder dry yeast Dinitrophenol Aspirin liguid nitro-gon Nitrogen gas cAMP radioimmunoassay Test Kit3. Instrument: digital thermometer ( SY-2 ) pain threshold detector ( GS-840) Jiquid scintillator(LS6500)Methods1. Fever induced by yeast: The animals were divided into six groups: control group, model group, positive drug group (200mg/kg) , three herb groups (217mg/kg, 435mg/kg, 870mg/kg). The experiment was carried out on ratmodels by using dry-yeast as pyretogenic agent. We choose three dosage of herb to observe the antipyretic effect of anti-fever 2 and aspirin as control.2. Fever induced by dinitrophenol; Group dividing is as same as experiment 1. Drugs were administered continuously for three days as follows: positive drug (100mg/kg) , three herb groups (109mg/kg, 217mg/kg, 435mg/kg) , control group (salt water) , model group (salt water). Then we began to make models. 3. Writhing test: The mice were divided into six groups randomly: control group, positive drug group ( 300mg/kg) , four herb groups ( 742mg/kg, 1484mg/kg, 2986mg/kg, 5936mg/kg). The analgesic effect was determined with mouse writhing method of celiac acetum injecting.4. Hot plat test; Group dividing is as same as experiment 3. Through measuring the changes of pain threshold, we observed analgesic effect of anti-fever 2.5. Assay of cAMP content in hypothalamus of rats: Model building is the same as experiment 1. There were total four groups: control group, model group, two herb groups (217mg/kg, 435mg/kg). After 6 hours of yeast injection, rats were decapitated quickly and hypothalamus tissues were taken out and put into liquid nitrogen for fixation. Then we began to assay the cAMP content in hypothalamus according to specification of the Test Kit.ResultsIn the yeast inducing fever test compared with control group the changes of rats'temperature in model group are obvious (P <0. 001) . Compared with model group the changes of temperature in herb groups have statistical significance. Anti-fever 2 can significantly inhibit uprise of temperature in rats induced by yeast (P<0.01). The medial dosage (435mg/kg) has the best effect (P<0. 001). The durg can also prevent fever induced by dinitrophenol. In analgesic studies using hot plat test and writhing test on mice anti-fever 2 has obvious inhibition of ache induced by acetum(P <0. 001) and can raise pain threshold of mice on hot plat evidently ( P <0.01). Compared with control group the anti-fever can inhibit the rise of cAMP content in the hypothalamus of rats ( P < 0. 05).Conclusion1. Our test has proved that anti-fever 2 has significant antipyretic effect on fevered rats induced by dry-yeast and dinitrophenol.2. In Writhing test and hot plat test anti-fever 2 has significant analgesic effect on model mice.3. Anti-fever 2 can distinctly inhibit the rise of cAMP content in hypothala-mus tissues of fevered rats.4. These facts suggest that anti-fever 2 possesses obvious antipyretic effect on fever rats and its action may be mediated by lowering cAMP level in hypothal-amus.
Keywords/Search Tags:anti-fever2, antipyretic, analgesic, cAMP
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