Effects Of DES On Apoptosis,NF-kB/p65 In HCC Cell Related To HBV

Objective:Approach the mechanism which DES prevent HCC from hepatitis B whether or not through inhibit the high express of NF-kB/p65 induced by HBxAg.Methods:Seed HepG2.2.15 and HepG2 cell line in 24 shadow mask by different concertration(1×10~4/ml,3×10~4/ml,10×10~4/ml ),determined the best culture concertration and time to add drug by cytometry.DES were dissolved with complete DMEM medium;divided into three conch(100μg/ml,200μg/ml,400μg/ml),contrasted by DDP(20μg/ml).And determined the effection of inhibition by MTT method.Every one of cell lines was divided into three group:DES group,DDP group,control group.Determined the expression strength of HBxAg within HepG2.2.15 cell line by immumofluorescent method,the expression of apoptosis within two cell lines by FCM and activity of NF-kB/p65 by ELISA.Results:The best culture concertration of two cell lines was 3×10~4/ml,and the beginning of exponential growth phase was after 48h culture.DES and DDP all have the effection of inhibition on muitiplication of HepG2.2.15 and HepG2.Contrast with control group,rate of S phase was enhanced as well as of G2/M phase was decreased.There was no significant deviation between DES and DDP.DES and DDP all can decrease the expression strength of HBxAg and the activity of NF-kB/p65,There was no significant deviation between DES and DDP too. HBxAg can stimulate the expression of NF-kB/p65.Conclusions:1 DES has the effection of inhibition on multiplication of HepG2.2.15,2 DES can decrease the expression strength of HBxAg,and has the identify effection on cell cycle and apoptosis of HepG2.2.15,3 DES can decrease the expression of activited NF-kB/p65 within HepG2.2.15.