Study On Toxicology And Anti-tumor Effect Of Ipomoea Batatas Poir Cv Anthocyanins

AIM:The aim of the present study was to investigate the toxicity of Ipomoea Batatas Poir Cv Anthocyanins,and further to exam its anti-tumor activity.Therefore,to provide the experimental basis for anti-tumor medicine development.METHODS:By acute toxicity testing,Ames test, micronucleus test,CHL chromosomal aberration test and subchronic toxicity testing,the toxicities of Ipomoea Batatas Poir Cv Anthocyanins were detected.The anti-tumor effects of Ipomoea Batatas Poir Cv Anthocyanins were observed on the well-established S₁₈₀ tumor mice model and hepatoma H₂₂ mice model.RESULTS:1.Mice were treated by Ipomoea Batatas Poir Cv Anthocyanins(intragastric administration,21.7g/kg.bw).After 14 days,no toxic signs were detected.We concluded that Ipomoea Batatas Poir Cv Anthocyanins has no toxicity according to the acute toxicity classification.2. The reverse strain clones induced by different concentrations of Ipomoea Batatas Poir Cv Anthocyanins were less than twice of the control group.No dose-dependent action was observed.This result indicated that Ipomoea Batatas Poir Cv Anthocyanins in 0.2-5000μg has no gene-mutation effect.3.No significance was observed between the different concentrations of Ipomoea Batatas Poir Cv Anthocyanins treatment group and control group in the bone morrow micronucleus test,which indicated that Ipomoea Batatas Poir Cv Anthocyanins has no inhibitory and toxic action on bone morrow cells.4.The Ipomoea Batatas Poir Cv Anthocyanins induced chromosomal aberration was less than 5%in the CHL chromosomal aberration test,no significant deference was detected,which showed that Ipomoea Batatas Poir Cv Anthocyanins could not induce chromosomal aberration.5.In the subchronic toxicity testing,the body weight,food utilization rate, blood property and the size of viscera were also in the normal range in the Ipomoea Batatas Poir Cv Anthocyanins treatment groups.No pathological changes were observed in the morphology of liver, spleen,kidney,stomach,duodenum,testis and ovary.6.The tumor inhibitory rates in sacinoma S₁₈₀ mice model were 29.7,45.0 and 33.3%separately after high-dose Ipomoea Batatas Poir Cv Anthocyanins treated.While at the middle-dose of Ipomoea Batatas Poir Cv Anthocyanins,the tumor inhibitory rate was instable.The tumor inhibitory rate was less than 10%after low-dose of Ipomoea Batatas Poir Cv Anthocyanins treated.The tumor inhibitory rate in hepatoma H₂₂ mice model was instable compared with that of S₁₈₀ tumor mice model.CONCLUSION:Ipomoea Batatas Poir Cv Anthocyanins have no adverse effects in the experimental dosage used in this study,and it could have inhibitory effect on sacinoma S₁₈₀.