Studies On The Chemical Constituents And Biological Activities Of Euphorbia Antiquorum L.

The Euphorbia is the largest genus of the angiosperm which belongs to the Euphorbiaceae plants and includes 2000 species. They distribute all over the world, which are widely in the subtropics and the variable zone area. They are abundance in Africa and Central South America. In addition to 14 species which had been cultivated and naturalized, our country produces the approximate 66 original species. The total species are 80. They distribute in the north and the south equally. However, they are widely in southwest of the Traverses Mountainous area and the northwest arid area. Euphorbia plant's root and root tuber are famous for their medicinal purposes. Euphorbia antiquorum L. is our country traditional Dai medicine, which belongs to the Euphorbia subgenus plant.This paper is divided into four chapters,the first chapter is the discussion of the spectrum data of abietane lactone compound. Abietane lactone haveα,β-unsaturated-γ-lactone ring,which is located in C-12 and C-13. They obviously showed anticancer, carcinogenicity and antibacterial activities.They primarily exist in Euphorbia, Verbenaceae, Leguminous and other plants, especially in the Euphorbiaceae plants. Domestic and foreign scholars focus on this kind compound due to an abundance of plant resources of this kind compounds.90 abietane lactones have been isolated from those plants and showed a wide variety of biological activity.The second chapter is the research on chemical constituents of Euphorbia antiquorum L..In our investigation of chemical constituents and biological activities of Euphorbia antiquorum L. (collected from Yunnan province of China), 18 compounds were isolated from EtOAc extract of Euphorbia antiquorum L. by normal-phase and reverse-phase silica gel column chromatography and HPLC etc. Among them, 13 compounds were elucidated on the basis of chemical and spectral methods. They were named as: antiquorine A (1),antiquorine B (2),ent-13S-hydroxy-16-atisene- 3,14-dione(3),taraxerol(4),antiquorine C (5),,9β,19-Cyclolanostan-3β-ol (6),ψ-Taraxastane-3,20-diol (7),3,3',4'-tri-O-methylellagic acid (8),3,3'-di-O-methylellagic acid (9),6,7,8-trimethoxyl-coumarin (10),7-hydroxy-6-methoxy-coumarin (11),Kaempferol-3-O-L-rhamnopyranoside (12),daucosterol(13). Compound (1), (2) and (5)were the new compounds.Compound (9),(11) and (12)were isolated from this plant for the first time.The third and fourth chapters are photoactivated antimicrobial activity of two coumarins 10 and 11 isolated from Euphorbia antiquorum L.,experiment and discussion.They showed no photoactivated antimicrobial activity against Staphylocus aureus and Bacillus subtilis in dark and UV.