Studies On The Chemical Constituents And Bioactivities Of Euphorbia Sikkimensis And E. Stracheyi

This dissertation reported the chemical constituents isolated from E. sikkimensisand E. stracheyi and their antiangiogenic activity towards zebrafish embryos,cytotoxicity against A549cell line.The roots of E. sikkimensis have been used to treat poisoning, malaria,rheumatism, and jaundice in Chinese traditional medicine. Phytochemicallyinvestigation of the aerial parts of this plant led to the isolation of39compounds,including1alkaloid,7phenolic compounds,2coumarins,6flavonoids,7diterpenoids,5triterpenoids,4steroids,4tocopherol derivatives and3fatty acids.Among which,5diterpenoids were new compounds (1–5). It is a systematic researchwork about the isolation, structure identification and pharmacological evaluation ofthe chemical constituents from E. sikkimensis; tocopherol derivatives and ergosterolperoxide were found in genus Euphorbia for the first time; the absolute configurationof jatropholane-type diterpene was determined by ECD calculation. The isolatedcompounds were tested for their bioactivities, and compounds2and13showedantiangiogenic activity in a zebrafish model, compounds1,22and23exhibitedcytotoxicity against A549cell line.The roots of E. stracheyi have been used for hemostasis, analgesia and muscleregeneration. Phytochemically investigation of this plant led the isolation of43compounds, including:1alkyl glucoside,10phenolic compounds,1coumarin,4flavonoids,4ionone glycosides,2sesquiterpenes,18diterpenoids,1triterpenoid and2steroids, Among them,3diterpenoids were new compounds (40–42). Numbers oftigliane and other type diterpenoids, ionone glycosides, sesquiterpenes were isolatedfrom the E. stracheyi for the first time;3rare diterpenoids were also identified. Astigliane diterpenoids exhibit remarkable cytotoxicity, the isolated compounds wereevaluated for their antiangiogenic activity only. None of the compounds exhibitedobvious activity.