Highly Content Screening And Efficient Preparation Of Active Component From Bamboo Leaves

Bamboo is perennial evergreen plant,belongs to Bambuaoidease Nees of the family poaceae.It has been used for food and medicines for a long time in China.There is a large body of evidence showing that bamboo leaves is rich in flavinoids and polysaccharides. It has many fine functions,such as anti-free radical,oxidation resistance and protecting vasular system etc.Nowadays,it has received broad attention from all over the world.In order to take full use of bamboo,the work had been done in the paper was focused on the method of determination and high content,especially the extration and purification for flavinoids and polysaccharides in bamboo leaves. The main results are as follows:HPLC and UV-VIS spectrophotometry were applied to detect the content of flavonoids in the experiment.and the results of the two detection were compared.The method of quantitative detection were established. NaNO2-Al(NO3)3-NaOH UV-VIS spectrophotometry was been common used.But it's result was higher than true. The method of HPLC was reliable and precise by taking Orientin and Isoviextin as the index ingredient.The content of flavonoids of 27 breeds of bamboo leaves were determined.It was concluded that the total flavonoids content of per gram dry bamboo leaves was between 5.0-13.3 milligram.The result was basically consilient with the reported result.The polysaccharides content of different breeds of bamboo leaves were determined.It was concluded that the polysaccharides content of per gram dry bamboo leaf was between 19.4-56.9 milligram.The breeds of Ph.glauca McClue, Dendrocauamopsis oldhami.(munro)Keng f, Ph.edulis(Carr)A.etC.Riv contentted more flavonoids and polysaccharides.They are good materials of industry.Phyllostachys Heteroclata Oliver contentted high flavonoids,and Sh.chinensis Nakai contentted a lot of polysaccharides. But they were not grow on every hedge and not good materials of industry.The content of polysaccharides in insenescent leaves was higher than young leaves.The content of flavonoids didn't match this rule.By good single factor selecting experiment and orthogonal design,the best technology for extracting bamboo leaves was established.The best waterbath extraction conditons was:60% ethanol solution,50℃,1:20 (w/v) of material and 1.5 hours per time.The order of affect actors was:solid-to-liquid ratio≥ethanol solution≥temperature≥time.The best ultrasonic extraction conditions was:60 % ethanol solution,1:15 (w/v) of material and 30 min per time.The order of affect actors was:time≥ethanol solution≥solid-to-liquid ratio.By reviewing for several Maro-reticular and Polyamide resins,it was founded that the optimal Maro-reticular resin was AB-8 for the purification of flavonoids. Flavonoids could almost be develop out by using 70 % ethanol solution. Ethanol was better than methanol as eluant.Ceramic membrane,ultra filtration and nanofiltration was adoptted to purify the extract of bamboo leaves.They can make the extract solution turning clarify and transparentted.When the volume of extract solution was about 90L,and concentration was 9.32g/L and 7.08g/L,It's function was fine for use. The flavonoids extract which had been treated by membrane can be purified to 15.8%-17.8% by resins. Polyamide had better effect than AB-8 in purifying flavonoids extract that had been treated by membrane.And it's result can be optimized to 40.35%. The polysaccharides extract which had been treated by membrane can be purified to 40.20% by high ethanol concentration.