Therapeutic Effects Of Gross Saponins Tribulus Terrestris On Myocardial Ischemia Reperfusion Injury In Rats

The most effective method of limiting acute myocardial ischemia necrosis is reperfusion. The effect of reperfusion itself may be associated with polymophonuclear leucocyte(PMN) accumulation,a burst of oxygen free radical production.,activation of inflammation and calcium overload,which may result in paradoxical cardiomyocyte dysfunction a phenomenon termed reperfusion injury.Studies on the new agents used to treat myocardial ischemia reperfusion are still a pivotal issue.In the situation of myocardial ischemia,SOD decreases and LPO increases with its toxic output-MDA increasing relatively.MDA is a kind of toxic substance,which can overoxidate cell membrane.Tribulus terrestris is also called Bai Jili and Ci Jili.It refers to the dry,ripe fruit of the plant caltrop.Its main chemical ingredients are saponins,ketone,alkaloid.The gross saponins of Tribulus terrestris(GSTT)was one of the effective group ingredients which was extracted from the whole plant of caltrop.It is a mixture of over 10 kinds of saponins,mainly steroid saponins.It is a powder and dissolvable in water.Domestic research materials showed that GSTT had the effect of lowering blood pressure and stimulating anti-hypoxia,sheeting blood vessels,lowering cholesterol,inhibiting fat deposition on arteries,myocardium and liver.Our study discussed the therapeutic mechanism of GSTT through setting up myocardial ischemia-reperfusion model in rats In this project,we take the molecular technology to explore the mechanism to its protective effects.We made ischemia reperfusion model in rats to study the effects of GSTT on cadiocyte morphology changes and detect the activity of SOD,MDA,NO,AST,LDH,CK in rats serum.As the result of that the changes of cadiocyte morphology was serious edema and cytoplasm was stained slightly and nuclei were contracted and strained deeply after reperfusion.GSTT(30mg·kg^-1,15mg·kg^-1)reduced cellular edema, maintained the cadiocyte morphology.After myocardial ischemia-reperfusion,content of AST,LDH,CK and MDA increased but SOD and NO depressed(P＜0.05).GSTT group (30mg·kg^-1,15mg·kg^-1) could decrease the content of AST,LDH,CK,MDA and increase SOD,NO(P＜0.05).GSTT can lessen the myocardial ischemia-reperfusion injury. All these findings suggest that GSTT has the therapeutic potential against myocardial ischemia-reperfusion injury in rats.Therapeutics effects may be related to inhibition of lipid oxidation,protection modulation of endogenous antioxidant enzyme activitie.