The Study On The Secondary Metabolites From Myxobacteria Strain TA And Endophytic Fungal Strain AF001,EYR61

In this study,the secondary metabolites of strain TA of myxobacteria, endophytic fungi strains AF001 and EYR61 were studied.Their secondary metabolites were separated and purified,structure of compounds were elucidated by extensive spectroscopic data analyses.In the same time,some compounds were elucidated by X-ray crystallographic,Infrared spectrum, UV-visible spectrum and Optical rotations analysis,and antimicrobe, anticancer activities were also be studied.The secondary metabolites of the strain TA were studied and five compounds TA7,TA9,TA10,TA11 and TA12 were obtained.They were all polyethers elucidated by ~1H-NMR analysis.We consider that they are foam suppressor which added during liquid fermentation.The strain AF001 is an endophytic fungi of sweetsop,it was identified by morphological and molecule biological methods.The result indicated that the strain AF001 belonged to Guignardia mangiferae.The secondary,metabolites of its solid fermentation were studied,we got LAF15 and LAF16 two fatty acids and one sterols compound LAF18,moreover we found five new compounds LAF1,LAF4,LAF11,LAF12 and LAF13,these new compounds were elucidated by Infrared spectrum,UV-visible spectrum,Optical rotations analysis,and their antimicrobe,anticancer activities were also be studied,and we also did X-ray crystallographic analysis of compound LAF1.The result indicated that LAF4,LAF11 and LAF13 exhibited weak antimicrobe activities against Candida albicans at 50μg/mL,their suppression ratio were 12.9%, 33.7%and 21.5%respectively,five new compounds all exhibited weak anticancer activities against human liver cancer cell HepG2 at 20μg/mL,their suppression ratio were 11.35%,8.88%,6.71%,4.34%and 5.03%respectively.The strain EYR61 is an endophytic fungi of redpine,it was identified by morphological and molecule biological methods.The result indicated that the strain EYR61 belonged to Thermoascus crustaceus.Two compounds EYR1 and EYR15 were identified from secondary metabolites of EYR61 solid fermentation.EYR1 was a new compound,and the structure of EYR3,EYR7, EYR8 and EYR13 are being analyzed now.In conclusion,endophytic fungi,especially from offcinal,can produce numerous new and unique stucture secondary metabolites,and many of them have biological activity,so the secondary metabolites of endophytic fungi are an important source of new drug discovery.