The Experimental Study Of Endostatin Inhibited Hepatocellular Carcinoma In Nude Mice Tumor And Expression Of Their VEGF/VEGFR And UPA/uPAR

Objective: To study mechanism of endostatin influenced on hepatocellular carcinoma by its inhibiting proliferation of SMMC-7721 human hepatocellular carcinoma cells of nude mice planting hepato-cancer and expression of their VEGFmRNA,VEGF-CmRNA,VEGFR-1mRNA,VEGFR-3mRNA,uPAmRNA and uPARmRNA.Methods: An animal model was established through inoculating subcutaneouslt human SMMC-7721 hepatocellular carcinoma cells into nude mices.The effect of tumor growth of nude mice bearing cancer in different densities of endostatin was detected.The expression of VEGFmRNA, VEGF-CmRNA,VEGFR-1mRNA, VEGFR-3mRNA,uPAmRNA and uPARm- RNA in human SMMC7721 hepatocellular carcinoma cells and tumor tissue of nude mice planting cancer was detected by RT-PCR.Results: In vivo,the tumor weight and volume of nude mice planting cancer in Endostatin groups was smaller than Controlled group(p<0.05), the expression of VEGFmRNA,VEGF-CmRNA,VEGFR-1mRNA,VEGFR-3mRNA, uPAmR- NA and uPARmRNA in endostatingroups was lower than controlled group (p<0.05).Conclusions: Endostatin has a sigificant depressant dffect on the tumor growth of mude mice planting hepatocellular carcinoma,Endostatin may inhibit hepatocellular carcinoma cells by inhibiting the expression of VEGFmRNA, VEGF-CmRNA, VEGFR-1mRNA, VEGFR-3mRNA, uPAmRNA and uPARmRNA.