Expression Of Cyclin G1 In Cervical Carcinoma And Its Correlations With P27 And HPV

Objectives:①To study the expression and clinical significance of cyclin G1,cyclin G1 mRNA and P27 in normal cervical tissue,CIN tissue and cervical carcinoma tissue.Furthermore,the potential associations among them were analyzed.②To study the sub-genotypes and infection prevalence of human papillomavirus (HPV)in different patients with cervical lesions.And to discuss the potential relationship between cyclin G1 and HPV in various cervical lesions.Methods:①Immunohistochemistry was used to detect the expressions of cyclin G1,P27 and in situ hybridization was used to detect the expression of cyclin G1 mRNA in the 107 cases,which include 18 cases of normal cervical tissue, 43 cases of cervical intraepithelial neoplasia(CIN) and 46 cases of cervical carcinoma.More over,all the related clinicopathological informations were collected and analyzed.The potential correlation between cyclin G1 and P27 was also studied.②Flow-through hybridization and gene chip(HybrMax) was used to detect the sub-genotypes of HPV in the desquamated cervical epithelial cells and immunohistochemistry was used to detect the expression of cyclin G1 in the same patients,which include 20 cases of normal cervix uteri,44 cases of CIN and 28 cases of cervical carcinoma.The potential correlation between cyclin G1 and HPV was also studied.Results:①In normal cervical tissue,CIN and cervical carcinoma,the positive expression rates of cyclin G1 were gradually increased(the differences in each pairs of group were statistically significant,p＜0.05).The different expression of cyclin G1 mRNA between CIN and cervical carcinoma was not statistically significant(p＞0.05).However,both of them were obviously higher than it in normal cervical tissue(p＜0.05).The expression of cyclin G1 had positive correlation with the expression of cyclin G1 lmRNA.In cervical carcinoma,the expression of cyclin G1 had significant correlation with lymph-node metastasis,whereas no relationships were observed with other pathologic characters.While the expression of cyclin G1 mRNA had no relationships with all pathologic characters.②In normal cervical tissue,CIN and cervical carcinoma,the positive-expression rates of P27 were gradually decreased(p＜0.05).The expression of cyclin G1 had negative correlation with the expression of P27(r=-0.424, p=0.003).In cervical carcinoma,the expression of P27 had significant correlation with lymph-node metastasis,whereas no relationships were observed with other pathologic characters.③In normal cervical tissue,CIN and cervical carcinoma,the infection rates of HPV,HR-HPV and HPV16 were all gradually increased(p＜0.05).In cervical carcinoma,the first 6 common sub-genotypes of HPV were(in descending sequences) HPV16,33,18,31,52,58.④In cervical carcinoma,the expression rate of cyclin G1 had positive correlation both with the infection rate of HR-HPV(r=0.382,p=0.045) and with the infection rate of HPV16(r=0.519,p=0.005).Conclusions:①The overexpression of cyclin G1 protein and mRNA might play an important role in human cervical tumorigenesis.The cause of this overexpression might be related with increased cyclin Gl mRNA level.②The underexpression of P27 might play an important role in human cervical tumorigenesis.③The expression of cyclin G1 had negative correlation with the expression of P27,and both of them had significant correlation with lymph-node metastasis.So they might be used to evaluate tumor malignancy and prognosis of cervical carcinoma.④Infection of HR-HPV was the major cause of cervical carcinoma.The expression rate of cyclin G1 had positive correlation with the infection rate of HR-HPV in cervical carcinoma.A synergistic interaction between them might benefit to the tumorigenesis of human cervical carcinoma.