Relationship Of Biological Status Of HPV And Human TERC Gene Amplification In Cervical Lesions

Background and ObjectiveFrom a global point of view, Cervical cancer is one of the most common cancer in women, it’s incidence slightly lower than breast cancer.China’s new cases about131,500per year, accounting for18.8percent of the world’s new cases, and it was getting younger and younger.lt is reported that the infection of high-risk types of HPV (human papilloma virus) is one of the important initiation factor of the vast majority of cervical cancer in human,99.8%of patients with cervical cancer could be detected in high-risk type of HPV-DNA exists.The most of HPV infections are short-term infection, only a portion of HPV infections to the successful establishment of persistent infection and persistent HPV infection is the inevitable development of cervical cancer.The carcinogenic effects of HPV and HPV-DNA integration are related. HPV-DNA integration into the host genome will lead to a host cell to produce a series of irreversible chain reaction, including the tumor suppressor gene function defects, genome instability and cell immortalization.At present, HPV has been reported in more than120kinds,27kinds of which is found from the cervical carcinoma and cervical cancer is divided into two kinds of low-risk and high-risk.Among them, high-risk types include HPV-16,18,31,33,35,45and low-risk types include HPV-6,11,34,42,43,44and so on.At present, the clinical detection methods generally detect any HPV infection, but can not determine the HPV whether integration.Hopman think genomic integration of oncogenic HPV and gain of the human telomerase gene TERC appear to be important associated genetic events in the progression of uterine cervical dysplasia to invasive cancer.HPV infection and Hterc amplification in cervical cancer at home and abroad to carry out a more mature, but few human papilloma virus(HPV) integration of state and human telomerase RNA component gene(hTERC) amplification with cervical lesions relations reports.In this study, through the application of FISH and in situ hybridization, to explore the relationship of Biological status of HPV and human TERC gene amplification with Cervical lesions.Materials and Methods1Materials1collect paraffin-embedded tissue of cervical lesion in women attending the First Affiliated Hospital of Zhengzhou University from January2008to October2008.All specimens were confirmed by pathology diagnosis and I have the consent of the informed consent of patients.According to WHO(2003) standard diagnosis,114cases were divided into five groups, namely non-CIN group (including36cases of cervical chronic inflammation and normal cervix), CIN1group of10cases,16cases CIN2, CIN337cases,15cases of cervical cancer.The specimens were taken, all patients were radiotherapy, chemotherapy and other special treatment.Exclusion criteria:In addition to congenital genetic disease, pregnancy, acute inflammation of the reproductive tract and in patients with serious medical illness.2MethodsHTERC genetic testing:Application of fluorescence in situ hybridization (FISH) test hTERC gene amplification, the directly labelled probes for chromo-some3(3c), chromo-some7(7c), and the TERC region on3q26.3were hybridized in a mixture, the fluorescence signal is red, CSP3of DNA hybridization on the3rd chromosome centromere (3p11.1-q11.1), the fluorescence signal in green (cells in green). CSP3is control probe, TERC is detection probe.HPV biological state detection:Application of in situ hybridization detection methods of cervical HPV infection in the organization, and integrate the situation for testing.If hybrid signal is crumb, prompting the HPV-DNA in the free state;If a hybrid signal patch, prompting the HPV-DNA in a state of integration.Results1. HPV-DNA infection status:14cases of cervical lesions, the positive rate of HPV infection was71.92%.Linear trend test results suggest that HPV infection results in5samples, HPV infection-DNA of positive were33.3%,80.0%,87.5%,91.9%,93.3%.HPV infection positive rate gradually increased with increasing level of cervical lesions (X2=52.49,P＜0.001) HPV infection results in five groups of samples by pairwise comparison, the CIN group significantly lower than the CIN3group, the cervical cancer group;The CIN1group significantly lower than the CIN3group, the cervical cancer group;The CIN2group significantly lower than the CIN3troup, the cervical cancer group.2. HPV-DNA integrated situation:82cases of HPV infection-positive patients, the positive rate of HPV integration is52.44%.Linear trend test results suggest that HPV integration results in5samples, HPV-DNA integrated positie were0%,10.0%,18.8%,73.0%,80.0%.HPV integration positive rate gradually increased with increasing level of cervical lesions (X2=32.80,P＜0.001).HPV integration results in five groups of samples by pairwise comparison, the CIN group significantly lower than the CIN2group, the CIN3group, the cervical cancer group.3. HTERC gene amplification situation.114cases of cervical lesions, hTERC gene amplification-positive rate of45.61%.Linear trend test results suggest that hTERC gene amplification results in5samples, hTERC gene amplification positie were2.8%,10.0%,50.0%,78.4%,86.7%.HTERC gene amplification positive rate gradually increased with increasing level of cervical lesions (X2=55.56,P＜0.001) hTERC gene amplification results in five groups of samples by pairwise comparison, The CIN group significantly lower than the CIN2group, the CIN3group, the cervical cancer group;The CIN1group significantly lower than the CIN3group, the cervical cancer group.4. Relations of HPV-DNA infection, integration and hTERC amplification. Relations HPV-DNA infection and hTERC amplification, Non-CIN group McNemar test results indicate that the risk of HPV infection is higher than the chance of hTERC gene amplification, and kappa agreement test also shows that the positive rate of HPV infection and hTERC gene amplification.Relations HPV-DNA integration and hTERC amplification, five groups of samples by the McNemar test results were not statistically different, HPV-DNA integration situation and hTERC gene amplification in line, and Kappa agreement test are two ways to predict the effect of cervical cancer.Conclusions1. CIN1/2as a low-level intraepithelial neoplasia, HPV-DNA is still not integrated into the host chromosomes, but also HPV infection after an early event in the malignant transformation of the host, and CIN3as a high-level intraepithelial neoplasia is often integrated with the HPV-DNA is closely related to malignant transformation close.2. The exception of the hTERC gene amplification rate was gradually increased with the level of progress of cervical lesions, it hints hTERC gene amplification and abnormal cervical lesions levels.3. HPV-DNA integration and hTERC gene amplification, HPV-DNA integration and hTERC amplified the effect of cervical cancer predicted consistent.