Study On Relationship Between Myeloperoxidase-463G/A Polymorphismsand Coronary Heart Disease

Background:Myeloperoxidase(MPO) and its inflammatory cascade represents an attractive target for prognostical investigation and therapeutics in atherosclerotic cardiovascular disease. Existing researches have revealed that the level of MPO in blood serum is related closely with coronary heart disease(CHD) and MPO-463G/A polymorphisms is correlated with the susceptibility of CHD.But there are few reports on the relationship between MPO-463 polymorphisms and the severity of coronary artery lesions. The relationship of MPO-463 polymorphisms with coronary artery in-stent restenosis has not reported yet. In present study we focused on the above questions.Methods:We performed case-control study. Patients were divided into CHD group(case group) and healthy group(control group) judged by coronary angiography. The group of CHD was further divided into four types of sub-groups(stenosis 50-75% group and stenosis >75% group) according to the extent of the severity of coronary artery lesions. the patients of CHD groups that received percutanous coronary intervention (PCI) were also divided into in-stent restenosis group and non-restenosis group according to the angiographic follow-up results. According to the results of the genotype of different groups, the relationship of MPO-463 polymorphisms with the severity of coronary artery lesions was analyzed; the relationship of MPO polymorphisms with coronary artery in-stent restenosis was also studied;PCR-RFLP method was used to decide the genotype of patients. SPSS 13.0 software package was used for statistical analysis.Results:In healthy group, the gene frequency of AA allele is 0.057 , 0.524 for GG allele; In CHD group, the gene frequency of AA allele is 0.014, 0.672for GG allele, which has significant statistic difference (χ2=9.55 P<0.05). The incidence rate of CHD of patients with AA allele was 0.186 times of that of those without GG allele, which has significant statistic difference(χ2=6.585 , p=0.01 , OR=0.186, 95%CI0.045-0.769).Among the CHD group, There are no significant difference between in-stent restenosis group and non- restenosis group.There are no significant difference between two sub groups of CHD groups.Conclusions: 1. We found that 463G/A polymorphisms of the MPO gene influences the risk of CHD. The A allele had protective function in CHD occurrence.The risk of CHD for carrying GG allele was obviously higher than carrying A allele.2. When the stenosis of coronary artery is equal to or more than 50%,, the severity of coronary artery lesions has no significant difference with MPO-463G/A polymorphisms.3. There were no significant difference in genotype between in-stent restenosis group and non-restenosis group, indicating that in-stent restenosis is not correlated with the 463G/A polymorphisms.