Investigation Of Hezhongxiaokui Decoction's Protective Effect On Gastric Mucosa Of The Rats With Ulcer Induced By Acetic Acid

Objective:To investigate the protective effect of Hezhongxiaokui decoction on gastric mucosa of rats with ulcer induced byAcetic Acid.To study its therapeutic effect as well as the possible mechanism.And to provide a clinical rationale for treatment of gastric ulcer.Methods:10 of 70 male healthy adult rats from quality,range 200—250g,were choosed to form the normal control group at random. The others were all induced chronic gastric ulcer by Acetic Acid,(inject 30μl 100%Acetic Acid at the Subserosa of juncture of the ventral aspect corpus gastricum of stomachus glandularis and gastric antrum to form a white nodule with approxinate diameter of 3mm ).At the 4th day after modiing 10 rats were choosed to evaluate whether the modle was successful.If success rates was greater than 80%,successful modle was seen established.The left 50 rats were devided into 5 groups at random:Model group,high dose group of Hezhongxiaokui decoction(25.2g/Kg*d),medium dose group of Hezhongxiaokui decoction(12.6g/Kg*d),low dose group of Hezhongxiaokui decoction(6.3g/Kg*d),Lizhu Dele group(0.36g/Kg*d). Rats of Hezhong Xiaokui decoction group and Lizhu Dele group were intragastric administrated Hezhong Xiaokui Physic liquors and Lizhu Dele Physic liquors respectively,one time per day,totally 16 days.The others were intragastric administratednormal saline. 16 days later,All the rats were executed to collect blood to detect the content of Plasma ET,and rats' s belly were cut open and removed the stomach to slice the scar of ulcer tissue.After HE dyeing and PAS dyeing separately we observed the healing condition of the ulce,r and measured the thickness of regenerative mucosa,Meanwhile, we detect the content of NO,MDA,SOD,EGF,6-Keto-PGF,a of stomach tissue.At last we compared the difference of interclass and analyzed data by SPSS statistics software.Results:1.The determination the thickness of theregeneratice mucosa on chronic gastric ulcer rats has showed that compared with model group, the thickness of gastric mucosa increased significantly in each dose of Hezhong Xiaokui group and Lizhu Dele group(P<0.05-0.01), High dose group of Hezhongxiaokui decoction had muchthicker regeneratice mucosa than Lizhu Dele group,and there was remarkable difference between the high dose group and the LizhuDelegroup (P<0.05).and there was no significant difference between the high dose group and the normal control group(P>0.05).On increasing the thickness of the regeneratice mucosa on chronic gastric ulcer rats there's a dose-dependent effect in the result. 2.The determination of the content of SOD and NO has showed that the content of SOD and NO of each dose group of Hezhongxiaokui decoction is much higher than that of LizhuDele and model group,and there was remarkable difference betweenhigh,medium,low dose group and LizhuDele group and model group(P<0.05-0.01).There was no significant difference between normal group and high dose group as well as the model group and LizhuDele group(P>0.05).On increasing the content of SOD and NO,there's a dose-dependent effect in the result.3.The determination of the content of ET and MDA has showed that the content of ET and MDA of each dose group of Hezhongxiaokui decoction is much lower than that of LizhuDele and model group, there was remarkable difference betweenhigh,medium,low dose group and LizhuDele group and model group(R<0.05-0.01).There was no significant difference between normal control group and high dose group as well as the model group and LizhuDele group(P>0.05). On decreasing the content of SOD and NO?there's a dose-dependent effect in the result.4.The determination of the content of EGF and 6-Keto-PGF₁a has showed that the content of EGF and 6-Keto-PGF₁a of each dose group of Hezhongxiaokui decoction and LizhuDele group is much higher than that of model group,and the content of 6-Keto-PGF₁a of LizhuDele group is much higher than that of each dose group of Hezhongxiaokui decoction(P<0.01).while the EGF a little higher(P>0.05).On increasing the content of SOD and NO,there' s a dose-dependent effect in the result.Conclusions:Hezhongxiaokui decoction can pronote the growth of the regeneratice mucosa on rats to accelerate the ulcer healing up and the effect was better thanLizhu Dele.the effect in the result was dose-dependent.It might be due to improvement of the the level of SOD,NO,EGF,6-Keto-PGF₁a and its decrease of the ET and MDA levels.Enhancing the protective effect on gastric mucosa was one of possible mechanisms on Hezhongxiaokui decoction treating gastric ulcer.The study provide a reference data for understanding the mechanism of this therapeutic method.Meanwhile,it is valuable for guiding the clinical practice in treating gastric ulcer and development of new medication.