The Active Components Of Prunella Vulgaris

The active antitumor components from Prunella vulgaris was Systematical studied, and optimized the techniques of the extraction and purified of the effective constituents. The contents of the study include: 1. the study of extraction technology; 2. The study of refined technology; 3. The study of quality standard; 4. the activity of the effective constituents was studied. In the extraction technology, the effect of factor like extract solvent was first studied. By active indicators of Oleanolic acid,Ursolic Acid,total flavonoids,total saponin. The optimal conditions for the extraction of Prunella vulgaris were determined to be 70% alcohol. By orthogonal test the extraction technology of extraction of Prunella vulgaris is screened. The extraction is refined with alcohol deposition. Adopting Oleanolic acid,Ursolic Acid,total flavonoids,total saponin as makers, optimum extraction was investigated by orthogonal design. The extraction was refined using macroporous absorption resin. By orthogonal test, the refined technology of Prunella vulgaris is screened. The techniques of extraction and accumulation of the effective constituents were studied and more than 50% of the effective constituents were determined. The feasibility of the techniques was proved in the large experiments; the quality standard of the effective constituents for anti-tumor was studied and the quality of the effective constituents was controlled by taking the Oleanolic acid,Ursolic Acid,total flavonoids,total saponin as indexs and the quality stability of the effective constituents in Prunella vulgaris was ensured;the activity of the effective constituents in Prunella vulgaris was studied preliminary by taking anti-tumor as indexs, the results showed that the effect of the effective constituents in Prunella vulgaris exhibited highly anti-tumor actions. The results of the research contributed to the declaration of taking the effective constituents in Prunella vulgaris as material for the Fith New Drug.