The Study Of Preparation Process And Pharmacodynamics On Subhealth Tongbian Herbal Tea

Subhealth Tongbian herbal tea in the South Medical University, Professor Luo Ren, investigating subhealth crowd in the large sample epidemiological survey based on the combination of traditional Chinese medicine basic theory and clinical experience over the years, in view of the crowd subhealth functional constipation symptoms, design herbal tea beverages.Subhealth crowd functional constipation, a higher rate of incidence than the normal, in Guangdong Province subhealth investigate, there is constipation symptoms has 1386 cases, in the sub-health population incidence rate of 34.4%. Western medicine for the treatment of functional constipation main bulk agents, osmotic agents and irritant laxative purge, recent studies suggest that prostaglandin-type drugs are also effective treatment of constipation, but the overall effect of poor treatment, and side-effects inevitable. Chinese medicine through the Treatment usually can achieve better efficacy, but the use of drugs to interfere with constipation, patients in subhealth unacceptable.Drinking herbal tea in time to add liquid and more universally accepted therapeutic health traditions and customs in our country, it has good practical value.This thesis through to constipation as the main syndromes of subhealth population epidemiological survey, which summed up the crowd constipation-subhealth distribution characteristics of TCM Syndrome, according to the main syndromes Designed prescription. And then proceed to the herbal preparation process, pharmacology studies, herbal tea laxative subhealth industrial production to provide a scientific basis. This is where our choice of the head. In the development into the effectiveness of herbal drinks and preparations before they make a preliminary evaluation process is essential. In this experiment include epidemiological investigation, preparation technology research, pharmacological research three aspects.PartⅠSubhealth constipation epidemiological studiesObjective: to constipation as the main crowd Syndromes subhealth epidemiological investigation, analysis constipation-subhealth TCM Syndromes distribution, so as to subhealth constipation herbal tea group to provide a scientific basis.Methods: A cross-sectional study methods, the use of the site survey, so that the survey questionnaire to fill subhealth, combined with the results of clinical examination to determine health status (including health, sub-health status, disease). At the same time, through the physician survey interviews collected TCM four diagnostic information, according to the TCM Syndrome Differentiation Differentiation standards. Then, the above-mentioned questionnaire qualified by information after the time of admission to treatment Epidata3.20 database, application software SPSS13.0 descriptive analysis to study the sub-health status of constipation-morbidity, the distribution of TCM Syndrome.Results: 1, investigated a total of 861 medical colleges and universities, such as enterprise specific group of people, has 3981 people in the sub-health status, prevalence rate of 65.2 percent, with constipation as the main clinical manifestations of the population accounted for 34.9 percent of sub-health. 2, the survey results show that constipation-subhealth TCM is mainly expressed in Yin deficiency, Qi Deficiency and Qi stagment.PartⅡThe second part of subhealth herbal laxative preparation processObjective: To observe the water extraction and alcohol precipitation method, ultracentrifugation, ZTC1+1-Ⅱnatural clarifying agent on subhealth herbal laxative effect of the different treatment, look for the best crafts, herbal teas for subhealth Tongbian industrial production scientific basis.Methods: subhealth Tongbian prescription herbal medicine, add ten times the volume of water, decocted 2 times 2 hours, the combined filtrate, concentrated to 1:30 (original drug: extract). Water extraction and alcohol precipitation method using 95% ethanol to liquid ethanol concentration of 90%, standing 12 hours later, the siphon, leaching supernatant, and 60℃at the roundabout after ethanol Recycling pan, take the supernatant; Ultracentrifugation to liquid placed inside the speeding refrigerated centrifuge, 20℃for 5min after the rainbow 3000R centrifuge supernatant lessons; ZTC1+1-Ⅱclarifying agent natural law, according to product requirements configuration A, B components, will be 6% liquid volume of component B solution add liquid slowly trickle in, mix, and then 8% of liquid volume of component A slow trickle of add liquid, mix, and placing 60 - 70℃in a water bath holding 1 h, ambient temperature and then holding 24 h, after rough filtration centrifugation, the supernatant taken. The above three methods were to add pure water TC: liquid for 1:30. Following the above-mentioned high-temperature anti-virus solution, after standing in the sterile transparent glass, the ambient temperature holding for 30 days, the naked eye observation of the above-mentioned liquid color, clarity, the availability of particle wall situation. The best way to get from liquid for determination of total flavonoids.Results: ZTC1+1-Ⅱnatural law to clarify the best dose, speeding refrigerated centrifuge followed by aqueous alcohol the worst method.PartⅢSubhealth Tongbian herbal pharmacology researchObjective: To observe the herbal laxative subhealth on mouse small intestine motor function and bowel conditions, clearly the purge subhealth herbal laxative effect.Methods: SPF-class Kunming male mice 120, weight 18 - 22g, subhealth herbal laxative administration for low, middle and high three-dose group, doses are separately 1200,2400,3600 mg / kg. BW (equivalent to the human body, respectively, the minimum recommended amount of times 10,20,30), while constipation model based control group and negative control group. 12 animals in each group.1, small intestine exercise experiment: the experimental group according 1200,2400,3600 mg / kg. BW (both to the same volume) to give oral herbal laxative subhealth, both the control group given volume of distilled water, one time every day, give subhealth Tongbian herbal consecutive 7 days. Last evening the same day to the kind of fasting animals in each group (open water), 16 hours after the model control group and three dose groups were given a dose of 2mg/kg. BW's Compound Diphenoxylate Solution (0.2ml/10g.BW), negative control group given distilled water. 30 minutes after the three dose groups were given herbal tea containing subhealth Tongbian ink (including 5% of the activated carbon powder, 10% gum arabic), model control group and negative control group given ink. Given ink cervical dislocation 25 minutes after the animals were killed, open the abdominal cavity isolated mesenteric, pyloric cutting since the top, bottom to the ileocecal bowel, placed on trays and gently pull into a straight line to the small intestine were measured. Intestine length as "the total length of small intestine", from the pylorus to the forefront of ink to "promote the length of ink." Ink according to the following formula to promote the rate of (P), and data conversion (X) after the statistical analysis.2, the time of defecation, stool weight and fecal grains measured: the experimental group according 1200,2400,3600 mg / kg. BW (both to the same volume) to give oral herbal laxative subhealth, both the control group given volume of distilled water, one time every day, continuous drug delivery for 7 days. Last evening the same day to the kind of fasting animals in each group (open water), 16 hours after the model control group and three dose groups were given a dose of 10mg/kg. BW doses of Compound Diphenoxylate (0.2ml/10g.BW), negative control group given the same amount of distilled water. Diphenoxylate give compound 30 minutes after the model control group and negative control group of mice given gavage ink, 3-dose group with the corresponding ink subhealth herbal laxative at the same time start time. Each animal were reared separately, and the supply of feed and water, observed and recorded the first row of each animal and then time, 5 hours of defecation and weight of grains.3, the experimental data using statistical software SPSS13.0 ANOVA statistical treatment.Results: 1, sub-health of animal laxative herbal tea the general situation and weight the impact of herbal tea after Tongbian subhealth give each group of animals to normal diet and activity, the dose groups of mice droppings than the control group and did not appear soft thin it. Mice at the end of the experiment body weight and negative control group and model control group were not significant, show that the herbal tea laxative Subhealth weight gain in mice had no effect on the role.2, subhealth Tongbian herbal exercise of mouse small intestine constipation model the impact of the control group mice ink advance rate was significantly lower than negative control group, said the establishment of constipation model. High, medium and low dose group of mice was higher than that of the ink pushing forward model control group, by statistical analysis after data conversion, the invention of high, medium and low-dose group and constipation difference between the model group were significantly.3, subhealth Tongbian herbal tea on mouse defecation time, stool weight of grains and the impact of fecal constipation model control group of mice the first time will be greater than the negative control group, and fecal stool weight of the grains and were less than the negative control group, note the establishment of constipation model. Each dose group will be longer than the first model of the control group constipation shortened, high, medium and low dose group compared with their differences were significant ; High, medium and low dose group fecal constipation model grains are larger than the control group and model control group with constipation difference was significant; high, medium and low dose group is greater than the weight of the stool constipation model the control group and model control group with constipation difference was significant.Conclusion1, constipation subhealth crowd at a higher rate of incidence, the TCM Syndrome more scattered distribution, mainly concentrated in the syndrome of Yin deficiency, Qi Deficiency and Qi stagment.2, according to the epidemiological investigation and design of basic theories of Chinese medicine through herbal laxative subhealth Pharmacodynamic tests, there is specific purge function.3, different preparation process on the traits subhealth herbal laxative, stability, clarity have different effects, one of ZTC1+1-Ⅱnatural law clarifying agent was superior to aqueous alcohol and ultracentrifugation.