The Absorption Kinetics And Pharmacokinetics Of Puerarin And Ferulic Acid In Naodesheng Tablets

Select the Naodesheng tablet(embodied in Chinese Pharmacopoeia(2005) as a model drug.Naodesheng is a composite formula of TCM preparation consisting of five crude drug including Radix puerariae lobatae,Rhizome lignsticum chuanxiong, Panax pseudo-ginseng,Flos safflower and Fructus crataegi,which has been clinically used for the treatment of cerebral stroke and apoplexy linger effect.In this paper Naodesheng and its main active ingredient in rat metabolic characteristics was carried. And we also researched on Naodesheng active ingredient and its absorption characteristics in rat's intestinal.Major research findings and conclusions are as follows:1,Using HPLC to determination the main effective component's of Naodesheng and medicine extraction.The main effective component's medicine generation of dynamics experimental study provides the basis for the side to the medicament quantity The results show that the per gram Naodesheng tablets containg puerain 52.671mg/g,ferulic acid 0.85mg/g.The content of puerarin in Radix puerariae lobatae extract is 122.675mg/g,the content of ferulic acid in Rhizome lignsticum chuanxiong is 826.03μg/mL.2,Studies on pharmacokinetics of puerarin in pueraria and its compound preparation in rat(1) Has established in vivo sample analysis highly effective liquid chromatography method,carries on the blood plasma the pueraria content determination.After using the methyl alcohol subsidence protein,the direct specimen handling analysis,the flowing is the methyl alcohol-0.5%vinegar acid water(30:70),the examination wave length is 250nm.The linear relationship is 10～1000μg/L,correlation coefficient R=0.9993.High,middle and low concentration sample returns-ratio respectively is 89.90%,94.00%,95.39%.This method accurate reliable,convenient quickly,sensitivity high,specificity,has satisfied in this article analysis request. (2) Studied on the pharmacokinetic comparisons of puerafin after oral administration of radix puerariae lobatae aqueous extract and Naodesheng.It was found that the concentration-time data demonstrated that after radix puerariae lobatae aqueous extract and Naodesheng,the puerarin in the rat in vivo's medicine to move the process to conform to two compartmental models.No great difference in T_max.However, after Naodesheng group's C_max is 2.01times than radix puerariae lobatae aqueous extract group.And AUC_{0→∞} is 3.20times.3,Studies on pharmacokinetics of ferulic acid in pueraria and Naodesheng preparation in rat(1) Has established in vivo sample analysis highly effective liquid chromatography method,carries on the blood plasma the ferulic aciddetermination. After using the methyl alcohol subsidence protein,the direct specimen handling analysis,the flowing is the methyl alcohol-0.5%vinegar acid water(25:75),the examination wave length is 320nm.The linear relationship scope is 0.05～1μg/mL, correlation coefficient R=0.9994.High,the low concentration sample returns-ratio respectively is 102.50%,95.32%,97.20%.This method accurate reliable,convenient quickly,sensitivity high,specificity,has satisfied in this article analysis request.(2) Studied on the pharmacokinetic comparisons of ferulic acid after oral administration of monomer,radix chuanxiong aqueous extract and Naodesheng.It was found that the concentration-time data demonstrated that after monomer,radix chuanxiong queous extract and Naodesheng,the ferulic acid in the rat in vivo's medicine to move the process to conform to two compartmental models.The results showed that the compound can be combined to promote the absorption of ferulic acid, and enhance it's bioavailability in rats.4,A four-site perfused rat intestinal model was established.Studied on the intestinal absorption kinetics of puerarin(1) Inspected the pueraria element in 37℃the intestines perfusate stability,finally indicated thatn puerarin is stable during 24h,its RSD value is 4.51%.This to guarantee that the rat filled the class experiment's accuracy to provide the guarantee.(2) Studied on the rat intestinal absorption kinetics of puerarin in puerarin extract and Naodesheng.It was found that the absorption of puerarin on the duodenum and ileum,the absorption rate and lower.It was found that the absorption of puerarin on the duodenum and ileum,the absorption rate and lower.5,A four-site perfused rat intestinal model was established.Studied on the intestinal absorption kinetics of ferulic acid(1) Inspected the ferulic acid element in 37℃the intestines perfusate stability, finally indicated that the ferulic acid is stable during 24h,its RSD value is 4.40%.This to guarantee that the rat filled the class experiment's accuracy to provide the guarantee.(2) Studied on the intestinal absorption kinetics of ferulic acid monomer, extract Chuanxiong and Naodesheng.The results show that the intestinal absorption in rat of ferulic acid monomer,Chuanxiong extract and Naodesheng has no significant difference,and ferulic acid in the intestinal absorption of non-specific site.