Study In Situ On Rat Intestinal Absorption Mechanism Of Bazhen Decoction

The prescription of Bazhen Decoction originates from"Zheng Ti Lei Yao" written by Xue Ji. It is a Chinese traditional dosage which has a long history. It is the combination of Sijunzi and Siwu Decoction. Nowadays it is used generally in clinic, because it is representative prescription of invigorating vital energy and enriching the blood. Chinese herbal complex is high efficacy, low toxic side effects and by multi-channel and multi-target integration it play a regulating therapeutic role. So more and more specialists have paid attention to it. As a oral drug, the intestinal absorption which occupies an important position in medicine study is the first process to exert therapeutic effects, so my paper aimed at study the absorption mechanism. This paper study the absorption of the main components in Radix Angelica Sinensis, Ligusticum chuanxiong Hort, both of them and Bazhen Decoction in rat's intestines. The predominant content and result as followed:(1) The experiment analyzed the absorption mechanism of Ferulic acid in Radix Angelica Sinensis,Ligusticum chuanxiong Hort,both of them. Uptake of Ferulic acid depends on concentration and varies linearly, but the absorption rate of different concentration do not changed remarkably It effected the absorption consistented with first order kinetics, infered the process of absorption is passively diffusion. On environmental pH aspect, because Ferulic acid belongs to composition of weak acid and alkalies, the absorption is affected by environmental pH. On the choice of intestinal segments, most of the Ferulic acid is absorbed by the stomach. In order to identify whether the absorption is effected by the P-gp, we corporate Ferulic acid with inducer-rifampicin or inhibitor-verapamil together. The result manifests the absorption is effected by the P-gp. In the future clinic, we can use the Ferulic acid and inducer-rifampicin together to increase the biological effective.(2)The experiment analyzed the absorption mechanism of Peoniflorin and Ferulic acid in Bazhen Decoction. It is the same with Ferulic acid, the absorption of Peoniflorin and Ferulic acid in Bazhen Decoction are studied on four aspects such as concentration, pH, intestinal segments, P-gp. The absorption of Peoniflorin and Ferulic acid changed depend on concentration by linear relationship, the linear equations:Y=3.78X+1.1478(r=0.9974), Y=3.3485X+0.1457(r=0.9740). The absorption rate of different concentration do not changed remarkably, it reflected the absorption of Peoniflorin and Ferulic acid consistented with first order kinetics. It infered the process of absorption of Peoniflorin and Ferulic acid are passively diffusion. On pH aspects, the absorption of Peoniflorin is not affected by pH. On the absorption position, Ferulic acid in Bazhen Decoction the same as that in Radix Angelica Sinensis,Ligusticum chuanxiong Hort and both of them, the Ferulic acid absorption position in Bazhen Decoction is stomach too. Furthermore, we corporate Bazhen Decoction extact with inducer-rifampicin or inhibitor-verapamil together. The absorbed dose, Ka, absorption fraction and t1/2 of Peoniflorin and Ferulic acid have increased or decreased obviously. So we can include that Peoniflorin and Ferulic acid in Bazhen Decoction is affected by the P-gp, and in future clinic we can use with catastaltic of P-gp to increase its effective in Bazhen Decoction.(3) By compare absorption of Ferulic acid in Radix Angelica Sinensis, Ligusticum chuanxiong Hort, both of them and Bazhen Decoction compound, we can gain the result that Ferulic acid in Bazhen Decoction compound are absorbed more on the aspects concentration, pH, intestinal segments than in others. So Bazhen Decoction compound exploitation is valuable.